Citation:
Vinegoni C, Pitsouli C, Razansky D, Perrimon N, Ntziachristos V. In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography. Nat Methods. 2008;5 (1) :45-7.
 | 2008_Nat Meth_Vinegoni.pdf | 271 KB |
| Supplement.pdf | 644 KB |
Date Published:
2008 JanAbstract:
We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time.
See also: Research Article
