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Howard Hughes Medical Institute
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| DATE | PRESENTERS | PAPERS |
|---|---|---|
| TBD | TBD | TBD |
| DATE | PRESENTERS | PAPERS |
|---|---|---|
| 10/5/20 |
1) Annabel Sangree 2) Peter DeWeirdt |
1) Genetic screens in isogenic mammalian cell lines without single cell cloning https://www.nature.com/articles/s41467-020-14620-6 2) Optimization of AsCas12a for combinatorial genetic screens in human cells https://www.nature.com/articles/s41587-020-0600-6?proof=t |
| 9/28/20 |
1) Takashi Enomoto 2) Donghyun Lim |
1) Synthetic immunomodulation with a CRISPR super-repressor in vivo https://www.nature.com/articles/s41556-020-0563-3 2) Engineering designer beta cells with a CRISPR-Cas9 conjugation platform https://www.nature.com/articles/s41467-020-17725-0 |
| 9/21/20 |
1) Lucia Sereni 2) Devon Stork |
1) Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs https://www.nature.com/articles/s41467-020-16542-9 CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs https://www.nature.com/articles/s41467-020-17952-5 2) Total synthesis of Escherichia coli with a recoded genome https://www.nature.com/articles/s41586-019-1192-5 |
| 9/14/20 |
1) Hassan Bukhari 2) Liz Lane |
1) Cas9 activates the p53 pathway and selects for p53-inactivating mutations https://www.nature.com/articles/s41588-020-0623-4 2) Targeted In Situ Protein Diversification and Intra-organelle Validation in Mammalian Cells. https://www.sciencedirect.com/science/article/pii/S2451945620300684 |
| 6/1/20 |
1) Nirmalya Chatterjee 2) Li Li |
1) Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice https://www.cell.com/cell/fulltext/S0092-8674(20)30286-5 2) An Engineered CRISPR-Cas9 Mouse Line for Simultaneous Readout of Lineage Histories and Gene Expression Profiles in Single Cells https://www.cell.com/cell/fulltext/S0092-8674(20)30554-7 |
| 5/11/20 |
1) Shannon Knight 2) Enzo Mameli |
1) Genome-wide In Vivo CNS Screening Identifies Genes that Modify CNS Neuronal Survival and mHTT Toxicity https://linkinghub.elsevier.com/retrieve/pii/S0896-6273(20)30004-0 2) Massively parallel Cas13 screens reveal principles for guide RNA design https://www.nature.com/articles/s41587-020-0456-9 |
|
5/4/20 |
1) Jonathan Zirin 2) Stephanie Mohr |
1) Multiplexed conditional genome editing with Cas12a in Drosophila https://www.biorxiv.org/content/10.1101/2020.02.26.966333v1 2) Capturing RNA–protein interaction via CRUIS https://academic.oup.com/nar/article/doi/10.1093/nar/gkaa143/5788206 |
| 4/27/20 |
1) Baolong Xia 2) Navdar Sever |
1) Development of CRISPR as an antiviral strategy to combat SARSCoV-2 and influenza https://www.cell.com/pb-assets/products/coronavirus/CELL_CELL-D-20-00736... 2) In Utero Gene Editing for Monogenic Lung Disease https://stm.sciencemag.org/content/11/488/eaav8375 |
|
4/20/20 |
1) Tracy Zhang 2) Pedro Saavedra |
1) CRISPR–Cas12-based detection of SARS-CoV-2 |
|
4/13/20 |
1) Ram Viswanatha 2) Hassan Bukhari |
1) CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities https://www.nature.com/articles/s41586-020-2099-x 2) Fast and efficient generation of knock-in human organoids using homology-independent CRISPR–Cas9 precision genome editing https://www.nature.com/articles/s41556-020-0472-5 |
| 4/6/20 |
1) Russell Walton 2) Cole Peters |
1) Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants https://science.sciencemag.org/content/early/2020/03/25/science.aba8853.... 2) Immune-orthogonal orthologues of AAV capsids and of Cas9 circumvent the immune response to the administration of gene therapy. https://www.nature.com/articles/s41551-019-0431-2 |
|
3/30/20 |
1) Sarah Bowling |
1) A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA https://www.nature.com/articles/s41467-020-14977-8 2) CAMIO: a transgenic CRISPR pipeline to create diverse targeted genome deletions in Drosophila. https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa177/580... |
| 3/23/20 |
1) Ben Ewen-Campen |
1) A protocol for detection of COVID-19 using CRISPR diagnostics https://www.broadinstitute.org/files/publications/special/COVID-19%20det...(updated).pdf 2) GO: a functional reporter system to identify and enrich base editing activity https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa124/576... |
| 03/02/2020 |
1) Jon Rodiger 2) Gabriel Birchak |
1) Bradford J, Perrin D (2019) A benchmark of computational CRISPR-Cas9 guide design methods. PLoS Comput Biol 15(8): e1007274. https://doi.org/10.1371/journal.pcbi.1007274 2) Logsdon, G.L., Gambogi, C.W., Liskovykh, M.A., Barrey, E.J., Larionov, V., Miga, K.H., Heun, P., Black, B.E.: Human artificial chromosomes that bypass centromeric DNA. Cell 178: 624-639, 2019. https://www.ncbi.nlm.nih.gov/pubmed/31348889 |
| 02/10/2020 |
1) Patrick Griffin 2) Cole Peters |
1) CRISPR-engineered T cells in patients with refractory cancer https://science.sciencemag.org/content/early/2020/02/05/science.aba7365 2) Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. https://www.nature.com/articles/s41551-019-0501-5 |
| 01/27/2020 |
1) Erik Aznauryan 2) Ah-Ram Kim |
1) Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs. https://www.nature.com/articles/s41587-019-0387-5 2) A tunable orthogonal coiled-coil interaction toolbox for engineering mammalian cells. https://www.nature.com/articles/s41589-019-0443-y |
| 1/13/2020 |
1) Giulia Ceglie 2) Sara Nunes |
1) Highly efficient editing of the β-globin gene in patient-derived hematopoietic stem and progenitor cells to treat sickle cell disease. Nucleic Acids Res. Sep. 2019
2) De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens. Nat Commun. Oct 2019 |
| 12/09/2019 |
1) Charlotte Bellamy 2) Kyle McCracken |
1) MacLeod et al. Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells. Cell Rep. 2019 Apr 16;27(3):971-986.e9 2) Abudayyeh et al. A cytosine deaminase for programmable single-base RNA editing. Science. 2019 Jul 26;365(6451):382-386 |
| 12/02/2019 |
1) Ankita Singh 2) Zhenfei Xie |
1) Loveless et al. DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cells. bioRxiv. Sept. 29, 2019. 2) Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems https://www.sciencedirect.com/science/article/pii/S1097276519308020?via%... |
| 11/25/2019 | NO MEETING | – – – – – – – – |
| 11/18/2019 |
1) Shannon Knight 2) Enzo Mameli |
1) Tian et al. CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons. Neuron. 2019 Aug 5. pii: S0896-6273(19)30640-3. 2) Freije et al. Programmable Inhibition and Detection of RNA Viruses Using Cas13. Mol Cell. 2019 Oct 2. pii: S1097-2765(19)30698-7 |
| 11/11/2019 | NO MEETING (Holiday) | – – – – – – – – |
| 11/04/2019 |
1) Jonathan Zirin 2) Stephanie Mohr |
1) Lin et al. Microhomology based CRISPR tagging tools for protein tracking, purification, and depletion. J Biol Chem. 2019 May 28. 2) Smits et al. Biological Plasticity Rescues Target Activity in CRISPR Knockouts. Nat Methods. 2019 Oct 28. |
| 10/28/2019 |
1) Baolong Xia 2) Ben Ewen-Campen |
1) Anzalone et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21. 2) Ihry et al. p53 inhibits CRISPR-Cas9 engineering in human pluripotent stem cells. Nat Med. 2018 Jul;24(7):939-946. |
| 10/21/2019 |
1) Pedro Saavedra 2) Amit Choudhary |
1) Hanlon et al. High levels of AAV vector integration into CRISPR-induced DNA breaks. Nat Commun. 2019 Sep 30;10(1):4439. 2) Maji et al. A High-Throughput Platform to Identify Small-Molecule Inhibitors of CRISPR-Cas9. Cell. 2019 May 2;177(4):1067-1079.e19. |
| 10/14/2019 | NO MEETING (Holiday) | – – – – – – – – |
| 10/07/2019 |
1) Hassan Bukhari 2) Tracy Zhang |
1) Jayavaradhan et al. CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites. Nat Commun. 2019 Jun 28;10(1):2866. 2) Kemaladewi et al. A mutation-independent approach for muscular dystrophy via upregulation of a modifier gene. Nature. 2019 Aug;572(7767):125-130. |
| 09/30/2019 |
1) Ram Viswanatha 2) Cole Peters |
1) Pickar-Oliver et al. Targeted transcriptional modulation with type I CRISPR–Cas systems in human cells. Nat Biotechnol. 2019 Sept 23. 2) Villiger et al. Treatment of a metabolic liver disease by in vivo genome base editing in adult mice. Nat Med. 2018 Oct;24(10):1519-1525. |
| 09/23/2019 |
1) Afroditi Petsakou 2) Jun Xu |
1) Xu et al. CRISPR-Edited Stem Cells in a Patient with HIV and Acute Lymphocytic Leukemia. N Engl J Med. 2019 Sep 11. 2) Xu et al. Mass spider silk production through targeted gene replacement in Bombyx mori. Proc Natl Acad Sci U S A. 2018 Aug 28;115(35):8757-8762. |
| 09/16/2019 |
Justin Bosch Sarah Bowling |
1) Strecker et al. RNA-guided DNA insertion with CRISPR-associated transposases. Science. 2019 Jun 6. pii: eaax9181. 2) Dong et al. Genome-Wide Off-Target Analysis in CRISPR-Cas9 Modified Mice and Their Offspring. G3 (Bethesda). 2019 Sep 6. pii: g3.400503.2019 |
| 09/09/2019 |
Navdar Sever Ah-Ram Kim |
1) Quadros et al. Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins. Genome Biol. 2017 May 17;18(1):92. 2) Wang et al. Programmed chromosome fission and fusion enable precise large-scale genome rearrangement and assembly. Science. 2019 Aug 30;365(6456):922-926. |
|
07/01/2019- |
No Meetings | – – – – – – – – |
| 06/24/2019 |
Claire Hu Ricky Brathwaite |
1a) Dandage et al. beditor: A Computational Workflow for Designing Libraries of Guide RNAs for CRISPR-Mediated Base Editing. Genetics. 2019 Jun;212(2):377-385. 1b) Erard et al. A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout. Mol Cell. 2017 Sep 21;67(6):1080. 2) Dolan et al. Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Mol Cell. 2019 Apr 5. pii: S1097-2765(19)30217-5. |
| 06/17/2019 | NO MEETING | – – – – – – – – |
| 06/10/2019 |
Josh Li Zhenfei Xie |
1) Meltzer, et al. Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila. Nat Commun. 2019 May 8;10(1):2113. 2) Li, et al. CRISPR–Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol. 2018 Nov;20(11):1315-1325. |
| 06/04/2019 |
Stephanie Mohr Shannon Knight |
1) Port et al. A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila. bioRxiv May 13, 2019. 2) Back et al. Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats. Neuron. 2019 Feb 8. pii: S0896-6273(19)30062-5 |
| 05/27/2019 | NO MEETING | – – – – – – – – |
| 05/20/2019 | NO MEETING | – – – – – – – – |
| 05/13/2019 |
Julian Grunewald Clarita Ingaramo |
1) Grunewald J, et al. Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. 2) Pan et al. Near-infrared upconversion-activated CRISPR-Cas9 system: A remote-controlled gene editing platform. Sci Adv. 2019 Apr 3;5(4):eaav7199. |
| 05/06/2019 |
Jonathan Zirin Ben Ewen-Campen |
1) Hoffmann MD, et al. Cell-specific CRISPR-Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins. Nucleic Acids Res. 2019 Apr 15. pii: gkz271. 2) Guichard A, et al. Efficient allelic-drive in Drosophila. Nat Commun. 2019 Apr 9;10(1):1640. |
| 04/29/2019 |
Justin Bosch Chiao-Lin Chen |
1) Kocak DD, et al. Increasing the specificity of CRISPR systems with engineered RNA secondary structures. Nat Biotechnol. 2019 Apr 15. 2) Bian WP, et al. A knock-in strategy for editing human and zebrafish mitochondrial DNA using mito-CRISPR/Cas9 system. ACS Synth Biol. 2019 Apr 10. |
| 04/22/2019 | NO MEETING | – – – – – – – – |
| 04/15/2019 |
Baolong Xia Tracy Zhang |
1) Ma Z, et al. PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components. Nature. 2019 Apr 3. 2) El-Brolosy MA, et al. Genetic compensation triggered by mutant mRNA degradation. Nature. 2019 Apr 3. 3) Iyer S, et al. Precise therapeutic gene correction by a simple nuclease-induced double-stranded break. Nature. 2019 Apr 3. |
| 04/08/2019 |
Cory Smith Ankita Singh |
1) Smith CJ, et al. Enabling large-scale genome editing by reducing DNA nicking. bioRxiv. Mar. 15, 2019. 2) Tuladhar R, et al. CRISPR/Cas9-based mutagenesis frequently provokes on-target mRNA misregulation. bioRxiv. Mar. 20, 2019. |
| 04/01/2019 |
Pedro Saavedra Ram Viswanatha |
1) Min YL, et al. CRISPR-Cas9 corrects Duchenne muscular dystrophy exon 44 deletion mutations in mice and human cells. Sci Adv. 2019 Mar 6;5(3):eaav4324. |
| 03/25/2019 |
Kendell Clement Afroditi Petsakou |
1) Clement K, et al. CRISPResso2 provides accurate and rapid genome editing sequence analysis. Nat Biotechnol. 2019 Feb 26. 2) Katrekar D, et al. In vivo RNA editing of point mutations via RNA-guided adenosine deaminases. Nat Methods. 2019 Feb 8. |
| 03/18/2019 | NO MEETING | – – – – – – – – |
| 03/11/2019 |
Benjamin Kleinstiver Henrique Camara |
1) Kleinstiver BP, et al. Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. 2) Wang XW, et al. A microRNA-inducible CRISPR-Cas9 platform serves as a microRNA sensor and cell-type-specific genome regulation tool. Nat Cell Biol. 2019 Feb 25. |
| 03/04/2019 | NO MEETING | – – – – – – – – |
| 02/25/2019 | NO MEETING | – – – – – – – – |
| 02/18/2019 | NO MEETING | – – – – – – – – |
| 02/11/2019 |
Julio Sainz de Aja Sarah Bowling |
1) Grunwald HA, et al. Super-Mendelian inheritance mediated by CRISPR-Cas9 in the female mouse germline. Nature. 2019 Jan 23. 2) Salvador-Martinez I, et al. Is it possible to reconstruct an accurate cell lineage using CRISPR recorders? Elife. 2019 Jan 28;8. pii: e40292. |
| 02/04/2019 | NO MEETING | – – – – – – – – |
| 01/28/2019 |
Ah-Ram Kim Enzo Mameli |
1) Matharu N, et al. CRISPR-mediated activation of a promoter or enhancer rescues obesity caused by haploinsufficiency. Science. 2019 Jan 18;363(6424). pii: eaau0629. |
| NO MEETING |
2018: 12/17, 12/24, 12/31 2019: 01/07, 01/14, 01/21 |
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| 12/10/2018 |
Henrique Camara Maria Clara Ingaramo |
1) Garcia-Marques J, et al. Unlimited genetic switches for cell-type specific manipulation. bioRxiv [Internet]. Nov. 14, 2018. 2) Farzadfard F, et al. Single-Nucleotide-Resolution Computing and Memory in Living Cells. bioRxiv [Internet]. Feb. 16, 2018. |
| 12/03/2018 |
Stephanie Mohr Justin Bosch |
1) Chong Z-S, et al. Pooled extracellular receptor-ligand interaction screening using CRISPR activation. Genome Biol. 2018 Nov 26;19(1):205. 2) Fueller J, et al. CRISPR/Cas12a-assisted PCR tagging of mammalian genes. bioRxiv [Internet]. Nov. 20, 2018. |
| 11/26/2018 |
Baolong Xia Tracy Zhang |
1) Sanson KR, et al. Up, down, and out: optimized libraries for CRISPRa, CRISPRi, and CRISPR-knockout genetic screens. bioRxiv [Internet]. July 2, 2018. 2) Shen MW, et al. Predictable and precise template-free CRISPR editing of pathogenic variants. Nature. 2018 Nov 7. |
| 11/19/2018 |
Johana Vásquez Chiao-Lin Chen |
1) Haney MS, et al. Identification of phagocytosis regulators using magnetic genome-wide CRISPR screens. Nat Genet. 2018 Nov 5. |
| 11/12/2018 | NO MEETING | – – – – – – – – |
| 11/05/2018 |
Pedro Saavedra Ram Viswanatha |
1) Wroblewska A, et al. Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens. Cell. 2018 Oct 18. |
| 10/29/2018 |
Afroditi Petsakou Ben Ewen-Campen |
1) Schmidt F, et al. Transcriptional recording by CRISPR spacer acquisition from RNA. Nature. 2018 Oct 3. |
| 10/22/2018 |
Justin Bosch Stephanie Mohr |
1) Akcakaya P, et al. In vivo CRISPR editing with no detectable genome-wide off-target mutations. Nature. 2018 Sep 12. |
| 10/15/2018 |
Baolong Xia |
1) Sharon E, et al. Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing. Cell. 2018 Sep 18. pii: S0092-8674(18)31118-8. |
| 10/08/2018 | NO MEETING | – – – – – – – – |
| 10/01/2018 |
Ankita Singh |
1) Swings T, et al. CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing. Nat Commun. 2018 Jun 8;9(1):2231. |
| NO MEETING | 09/04, 09/10, 09/17, 09/24 | |
|
08/27/2018 |
Pedro Saavedra |
1) Horlbeck MA, et al. Mapping the Genetic Landscape of Human Cells. Cell. 2018 Aug 9;174(4):953-967.e22. 2) Roche PJR, et al. Homology Directed Repair by Cas9:Donor Co-localization in Mammalian Cells. bioRxiv [Internet]. Aug. 6, 2018. |
|
08/20/2018 |
Afroditi Petsakou |
1) Kundert K, et al. Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs. bioRxiv [Internet]. May 15, 2018. |
| 08/06/2018 | Justin Bosch | 1) Kosicki M, et al. Repair of double-strand breaks induced by CRISPR-Cas9 leads to large deletions and complex rearrangements. Nat Biotechnol. 2018;36 (8) :765-771. |
Who are we?
Researchers at HMS (Post-docs, grad students, technicians, PIs, etc).
What do we talk about?
Genome engineering/gene editing – novel methods, technical issues (e.g. off-target analysis), ethical concerns, etc.
Why are we doing this?
To stay up to date on recent techniques, stimulate critical discussions, establish contacts for collaboration
When/where?
12:30 PM – 1:30 PM Mondays, Room 354 New Research Building (see schedule)
What’s the format?
Presenters choose what they present. 2x presentations per meeting (30min each). Usually, this is a single recent paper in the field, presented as a PowerPoint to show the figure panels. Presenters can present any topic/format they wish, such as multiple papers, reviews, general discussion, chalk talk, etc.
Do I have to read the paper?
No, but it’s recommended.
Will I have to present?
We hope you will! But this is not strictly required.
I’m scheduled to present, what do I do?
Find a paper/topic you are excited about! The topic can be related to your expertise, or not. We provide a list of suggested papers (below) but feel free to consider alternatives. *Send your paper/topic selection to the organizer by the Friday before your presentation.* When you present, 1) introduce yourself, 2) explain why you selected the paper/topic, and 3) give background on the topic. Feel free to cut out panels or entire figures if it improves the presentation. Keep your presentation aimed at a general audience - most people do not read the paper and some will not be familiar with your topic.
Questions? Want to join the mailing list? Want to present? Have paper suggestions? – Contact Justin Bosch (jabosch@hms.harvard.edu) (Perrimon Lab)
HMS Email Accounts: Click here to subscribe yourself to the mailing list.
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Orthogonal tuning of gene expression noise using CRISPR-Cas.
https://academic.oup.com/nar/article/48/13/e76/5849902
Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors
https://www.nature.com/articles/s41587-019-0393-7
A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing
https://www.nature.com/articles/s41587-020-0535-y
Dual base editor catalyzes both cytosine and adenine base conversions in human cells
https://www.nature.com/articles/s41587-020-0527-y
A single CRISPR base editor to induce simultaneous C-to-T and A-to-G mutations
https://www.biorxiv.org/content/10.1101/729269v1.abstract
Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response
https://www.sciencedirect.com/science/article/pii/S2211124720307002
Targeted Perturb-seq enables genome-scale genetic screens in single cells.
https://www.nature.com/articles/s41592-020-0837-5
Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs.
https://www.nature.com/articles/s41477-020-0670-y
Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs.
https://www.nature.com/articles/s41467-020-16542-9
CRISPR-TAPE: Protein-Centric CRISPR Guide Design for Targeted Proteome Engineering
https://www.embopress.org/doi/full/10.15252/msb.20209475
Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants
https://www.nature.com/articles/s41587-020-0537-9
Multilayered VBC Score Predicts sgRNAs That Efficiently Generate Loss-Of-Function Alleles
https://www.nature.com/articles/s41592-020-0850-8
Synergistic Gene Editing in Human iPS Cells via Cell Cycle and DNA Repair Modulation
https://www.nature.com/articles/s41467-020-16643-5
Direct-seq: Programmed gRNA Scaffold for Streamlined scRNA-seq in CRISPR Screen
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-02044-w
Massively multiplexed nucleic acid detection with Cas13
https://www.nature.com/articles/s41586-020-2279-8
Very fast CRISPR on demand
https://science.sciencemag.org/content/368/6496/1265.abstract
Development of CRISPR-Cas13a-based antimicrobials capable of sequence-specific killing of target bacteria
https://www.nature.com/articles/s41467-020-16731-6
Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning
https://www.sciencedirect.com/science/article/pii/S0092867420306322
CRISPR artificial splicing factors
https://www.nature.com/articles/s41467-020-16806-4
CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity
https://www.nature.com/articles/s41587-020-0555-7
Single-cell Lineage Tracing by Integrating CRISPR-Cas9 Mutations With Transcriptomic Data
https://www.nature.com/articles/s41467-020-16821-5
Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA
https://advances.sciencemag.org/content/6/25/eaaz4849?utm_source=TrendMD...
Repurposing type I-F CRISPR-Cas system as a transcriptional activation tool in human cells.
https://www.nature.com/articles/s41467-020-16880-8
CRISPR-assisted Detection of RNA-protein Interactions in Living Cells
https://www.nature.com/articles/s41592-020-0866-0
The Histone Chaperone FACT Induces Cas9 Multi-turnover Behavior and Modifies Genome Manipulation in Human Cells
https://www.sciencedirect.com/science/article/pii/S1097276520303993
Precise, Predictable Multi-Nucleotide Deletions in Rice and Wheat Using APOBEC-Cas9
https://www.nature.com/articles/s41587-020-0566-4
Programmable M 6 A Modification of Cellular RNAs With a Cas13-directed Methyltransferase
https://www.nature.com/articles/s41587-020-0572-6
Efficient Gene Editing of Human Long-Term Hematopoietic Stem Cells Validated by Clonal Tracking
https://www.nature.com/articles/s41587-020-0551-y
Small-Molecule Control of Super-Mendelian Inheritance in Gene Drives
https://www.sciencedirect.com/science/article/pii/S2211124720308226
Toward a translationally independent RNA-based synthetic oscillator using deactivated CRISPR-Cas
https://www.biorxiv.org/content/10.1101/2020.05.13.094730v1.abstract
A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity
https://science.sciencemag.org/content/369/6499/54.abstract
Decipher the complexity of cis-regulatory regions by a modified Cas9.
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235530
Upgraded CRISPR/Cas9 Tools for Tissue-Specific Mutagenesis in Drosophila
https://www.biorxiv.org/content/10.1101/2020.07.02.185652v1.abstract
Multilayered VBC score predicts sgRNAs that efficiently generate loss-of-function alleles
https://www.nature.com/articles/s41592-020-0850-8
Sequence-specific Prediction of the Efficiencies of Adenine and Cytosine Base Editors
https://www.nature.com/articles/s41587-020-0573-5
Targeted, efficient sequence insertion and replacement in rice
https://www.nature.com/articles/s41587-020-0581-5
Blackjack Mutations Improve the On-Target Activities of Increased Fidelity Variants of SpCas9 With 5'G-extended sgRNAs
https://www.nature.com/articles/s41467-020-15021-5
A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing
https://www.nature.com/articles/s41586-020-2477-4
A High-Throughput Small Molecule Screen Identifies Farrerol as a Potentiator of CRISPR/Cas9-mediated Genome Editing
https://elifesciences.org/articles/56008
High-fidelity SaCas9 Identified by Directional Screening in Human Cells
https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.30...
In Vivo Repair of a Protein Underlying a Neurological Disorder by Programmable RNA Editing
https://www.biorxiv.org/content/10.1101/2020.02.26.966820v1.abstract
Chromothripsis as an on-target consequence of CRISPR-Cas9 genome editing
https://www.biorxiv.org/content/10.1101/2020.07.13.200998v1.abstract
Optimization of AsCas12a for combinatorial genetic screens in human cells
https://www.nature.com/articles/s41587-020-0600-6
High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening
https://www.nature.com/articles/s41467-020-17209-1
CRISPR-CasΦ from huge phages is a hypercompact genome editor
https://science.sciencemag.org/content/369/6501/333.abstract
Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA
https://www.nature.com/articles/s41467-020-17411-1
Bi-FoRe: an efficient bidirectional knockin strategy to generate pairwise conditional alleles with fluorescent indicators
https://link.springer.com/article/10.1007/s13238-020-00747-1
Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment
https://www.nature.com/articles/s41467-020-17418-8
Enhancement of target specificity of CRISPR-Cas12a by using a chimeric DNA-RNA guide
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa605/587...
CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells
https://www.nature.com/articles/s41587-020-0609-x
New base editors change C to A in bacteria and C to G in mammalian cells
https://www.researchgate.net/publication/343089654_New_base_editors_chan...
Bidirectional titration of yeast gene expression using a pooled CRISPR guide RNA approach
https://www.pnas.org/content/117/31/18424.short
A programmable sequence of reporters for lineage analysis
https://www.nature.com/articles/s41593-020-0676-9
Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters
https://www.nature.com/articles/s41587-020-0614-0
DNA capture by a CRISPR-Cas9–guided adenine base editor
https://science.sciencemag.org/content/369/6503/566.abstract
CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos
https://www.biorxiv.org/content/10.1101/2020.01.13.904763v1.abstract
Engineering designer beta cells with a CRISPR-Cas9 conjugation platform
https://www.nature.com/articles/s41467-020-17725-0
In vitro Cas9-assisted editing of modular polyketide synthase genes to produce desired natural product derivatives
https://www.nature.com/articles/s41467-020-17769-2
Scarless engineering of the Drosophila genome near any site-specific integration site
https://www.biorxiv.org/content/10.1101/2020.08.13.249656v1.abstract
A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
https://www.nature.com/articles/s41467-020-17962-3
An in vivo KRAS allelic series reveals distinct phenotypes of common oncogenic variants
https://cancerdiscovery.aacrjournals.org/content/early/2020/07/24/2159-8...
CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs.
https://www.nature.com/articles/s41467-020-17952-5
Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs
https://www.nature.com/articles/s41587-019-0178-z
Controlled Cycling and Quiescence Enables Efficient HDR in Engraftment-Enriched Adult Hematopoietic Stem and Progenitor Cells
https://www.sciencedirect.com/science/article/pii/S2211124720310822
CRISPR-nRAGE, a Cas9 nickase-reverse transcriptase assisted versatile genetic engineering toolkit for E. coli
https://www.biorxiv.org/content/10.1101/2020.09.02.279141v1
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos
https://www.nature.com/articles/s41598-020-71412-0
Synthetic immunomodulation with a CRISPR super-repressor in vivo
https://www.nature.com/articles/s41556-020-0563-3
Background-suppressed live visualization of genomic loci with an improved CRISPR system based on a split fluorophore
https://genome.cshlp.org/content/early/2020/09/04/gr.260018.119.short?rss=1
Engineering multiple species-like genetic incompatibilities in insects
https://www.nature.com/articles/s41467-020-18348-1
Massively parallel kinetic profiling of natural and engineered CRISPR nucleases.
https://www.nature.com/articles/s41587-020-0646-5
Mutation-Independent Allele-Specific Editing by CRISPR-Cas9, a Novel Approach to Treat Autosomal Dominant Disease
https://www.sciencedirect.com/science/article/abs/pii/S1525001620302367
A Redox-Based Electrogenetic CRISPR System to Connect With and Control Biological Information Networks
https://www.nature.com/articles/s41467-020-16249-x Massively parallel assessment of human variants with base editor screens
https://www.biorxiv.org/content/10.1101/2020.05.17.100818v1
Pronuclear Microinjection during S-Phase Increases the Efficiency of CRISPR-Cas9-Assisted Knockin of Large DNA Donors in Mouse Zygotes
https://www.sciencedirect.com/science/article/pii/S2211124720306069
Multiplex enCas12a screens show functional buffering by paralogs is systematically absent from genome-wide CRISPR/Cas9 knockout screens
https://www.biorxiv.org/content/10.1101/2020.05.18.102764v1
A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects.
https://www.nature.com/articles/s41592-020-0832-x
Cas9 Activates the p53 Pathway and Selects for p53-inactivating Mutations
https://www.nature.com/articles/s41588-020-0623-4
A Cas9 With PAM Recognition for Adenine Dinucleotides
https://www.nature.com/articles/s41467-020-16117-8
Genome-wide CRISPR Screening Reveals Genes Essential for Cell Viability and Resistance to Abiotic and Biotic Stresses in Bombyx mori
https://genome.cshlp.org/content/early/2020/05/18/gr.249045.119
Parallel CRISPR-Cas9 screens clarify impacts of p53 on screen performance
https://elifesciences.org/articles/55325
Targeted In Situ Protein Diversification and Intra-organelle Validation in Mammalian Cells
https://www.sciencedirect.com/science/article/pii/S2451945620300684
Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing
https://www.sciencedirect.com/science/article/pii/S2211124720306422
Enhanced Golic+: Highly effective CRISPR gene targeting and transgene HACKing in Drosophila
https://dev.biologists.org/content/early/2020/05/24/dev.181974
Evaluation of Off-Targets Predicted by sgRNA Design Tools
https://www.sciencedirect.com/science/article/pii/S0888754319307086
BEON: A Functional Fluorescence Reporter for Quantification and Enrichment of Adenine Base-Editing Activity
https://www.sciencedirect.com/science/article/abs/pii/S1525001620301908
Targeted mRNA Demethylation Using an Engineered dCas13b-ALKBH5 Fusion Protein
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa269/582...
Analysis and minimization of cellular RNA editing by DNA adenine base editors.
https://advances.sciencemag.org/content/5/5/eaax5717
Massively Parallel CRISPRi Assays Reveal Concealed Thermodynamic Determinants of dCas12a Binding
https://www.pnas.org/content/early/2020/05/05/1918685117
Genetic interaction mapping and exon-resolution functional genomics with a hybrid Cas9–Cas12a platform
https://www.nature.com/articles/s41587-020-0437-z
Fitness effects of CRISPR/Cas9-targeting of long noncoding RNA genes
https://www.nature.com/articles/s41587-020-0428-0
Reply to: Fitness effects of CRISPR/ Cas9-targeting of long noncoding RNA genes
https://www.nature.com/articles/s41587-020-0431-5
Increasing the Efficiency and Targeting Range of Cytidine Base Editors Through Fusion of a Single-Stranded DNA-binding Protein Domain
https://www.nature.com/articles/s41556-020-0518-8
An Engineered ScCas9 With Broad PAM Range and High Specificity and Activity
https://www.nature.com/articles/s41587-020-0517-0
A Male-Biased Sex-Distorter Gene Drive for the Human Malaria Vector Anopheles Gambiae
https://www.nature.com/articles/s41587-020-0508-1
Endogenous CRISPR/Cas9 Arrays for Scalable Whole-Organism Lineage Tracing
https://dev.biologists.org/content/147/9/dev184481
Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope.
https://www.nature.com/articles/s41467-020-15892-8
Plants with genetically encoded autoluminescence
https://www.nature.com/articles/s41587-020-0500-9
Enhancement of homology-directed repair with chromatin donor templates in cells
https://elifesciences.org/articles/55780
Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
https://www.nature.com/articles/s41467-020-15845-1
Safety and feasibility of CRISPR-edited T cells in patients with refractory non-small-cell lung cancer
https://www.nature.com/articles/s41591-020-0840-5
CRISPR-Cas13 Inhibitors Block RNA Editing in Bacteria and Mammalian Cells
https://www.sciencedirect.com/science/article/abs/pii/S1097276520302252
Massively Multiplexed Nucleic Acid Detection Using Cas13
https://www.nature.com/articles/s41586-020-2279-8
CRISPR/Cas9 mediated genetic resource for unknown kinase and phosphatase genes in Drosophila
https://www.nature.com/articles/s41598-020-64253-4
ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons
https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.30...
Perturbing Proteomes at Single Residue Resolution Using Base Editing
https://www.nature.com/articles/s41467-020-15796-7
Systematic in Vitro Profiling of Off-Target Affinity, Cleavage and Efficiency for CRISPR Enzymes
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa231/582...
Enabling Large-Scale Genome Editing at Repetitive Elements by Reducing DNA Nicking
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa239/582...
Multiplex secretome engineering enhances recombinant protein production and purity
https://www.nature.com/articles/s41467-020-15866-w
Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice
https://www.sciencedirect.com/science/article/pii/S0092867420302865
Extinction of All Infectious HIV in Cell Culture by the CRISPR-Cas12a System With Only a Single crRNA
https://academic.oup.com/nar/article/doi/10.1093/nar/gkaa226/5819595
Rewiring of Endogenous Signaling Pathways to Genomic Targets for Therapeutic Cell Reprogramming
https://www.nature.com/articles/s41467-020-14397-8
Directed evolution of adenine base editors with increased activity and therapeutic application.
https://www.nature.com/articles/s41587-020-0491-6
Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies
https://www.cell.com/cell/fulltext/S0092-8674(20)30332-9?dgcid=raven_jbs_aip_email
Combinatorial Single-Cell CRISPR Screens by Direct Guide RNA Capture and Targeted Sequencing
https://www.nature.com/articles/s41587-020-0470-y
Quantification of the affinities of CRISPR-Cas9 nucleases for cognate protospacer adjacent motif (PAM) sequences.
https://www.jbc.org/content/early/2020/04/01/jbc.RA119.012239.abstract
CRISPR Screens in Cancer Spheroids Identify 3D Growth-Specific Vulnerabilities
https://www.nature.com/articles/s41586-020-2099-x
Therapeutic base editing of human hematopoietic stem cells
https://www.nature.com/articles/s41591-020-0790-y
Genetic Interaction Mapping and Exon-Resolution Functional Genomics With a Hybrid Cas9-Cas12a Platform
https://www.nature.com/articles/s41587-020-0437-z
Targeted nanopore sequencing with Cas9-guided adapter ligation
https://www.nature.com/articles/s41587-020-0407-5
Abundance of conserved CRISPR-Cas9 target sites within the highly polymorphic genomes of Anopheles and Aedes mosquitoes.
https://www.nature.com/articles/s41467-020-15204-0
Prime genome editing in rice and wheat
https://www.nature.com/articles/s41587-020-0455-x
Conditional Single Vector CRISPR/SaCas9 Viruses for Efficient Mutagenesis in the Adult Mouse Nervous System
https://www.sciencedirect.com/science/article/pii/S2211124720302667
Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
https://science.sciencemag.org/content/early/2020/03/25/science.aba8853
A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing.
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-01989-2
Cas9-Mediated Gene-Editing in the Malaria Mosquito Anopheles stephensi by ReMOT Control.
https://www.g3journal.org/content/early/2020/03/02/g3.120.401133.long
Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling.
https://www.pnas.org/content/117/11/5853.abstract
Fast and efficient generation of knock-in human organoids using homology-independent CRISPR-Cas9 precision genome editing.
https://www.nature.com/articles/s41556-020-0472-5
Bridge helix arginines play a critical role in Cas9 sensitivity to mismatches.
https://www.nature.com/articles/s41589-020-0490-4
A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA
https://www.nature.com/articles/s41467-020-14977-8
Programmed chromosome fission and fusion enable precise large-scale genome rearrangement and assembly.
https://science.sciencemag.org/content/365/6456/922
RNA-guided retargeting of Sleeping Beauty transposition in human cells.
https://elifesciences.org/articles/53868
OffScan: a universal and fast CRISPR off-target sites detection tool.
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-6241-9
Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
https://www.ncbi.nlm.nih.gov/pubmed/32160537
Capturing RNA-protein interaction via CRUIS.
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa143/578...
CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects.
https://www.jbc.org/content/early/2020/03/11/jbc.RA120.012933.abstract
CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway.
https://link.springer.com/article/10.1007%2Fs13238-020-00699-6
CRISPR-Cas12b enables efficient plant genome engineering.
https://www.nature.com/articles/s41477-020-0614-6
Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
https://www.nature.com/articles/s41467-020-15226-8
Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping
https://www.nature.com/articles/s41467-020-14957-y
Comprehensive In Vivo Interrogation Reveals Phenotypic Impact of Human Enhancer Variants.
https://www.sciencedirect.com/science/article/pii/S0092867420302087
Highly Parallel Profiling of Cas9 Variant Specificity.
https://www.sciencedirect.com/science/article/abs/pii/S109727652030143X
CAMIO: a transgenic CRISPR pipeline to create diverse targeted genome deletions in Drosophila.
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa177/580...
Deep learning improves the ability of sgRNA off-target propensity prediction.
https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-020-...
Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.
https://www.nature.com/articles/s41587-020-0414-6
Targeted nanopore sequencing with Cas9-guided adapter ligation.
https://www.nature.com/articles/s41587-020-0407-5
Continuous evolution of SpCas9 variants compatible with non-G PAMs.
https://www.nature.com/articles/s41587-020-0412-8
Synthetic CRISPR/Cas9 reagents facilitate genome editing and homology directed repair.
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa085/573...
CRISPR-Based Adenine Editors Correct Nonsense Mutations in a Cystic Fibrosis Organoid Biobank.
https://www.sciencedirect.com/science/article/pii/S1934590920300199
Multiplexed and tunable transcriptional activation by promoter insertion using nuclease-assisted vector integration.
https://academic.oup.com/nar/article/47/12/e67/5424073
MagnEdit-interacting factors that recruit DNA-editing enzymes to single base targets.
https://www.life-science-alliance.org/content/3/4/e201900606
SpCas9-NG self-targets the sgRNA sequence in plant genome editing.
https://www.nature.com/articles/s41477-020-0603-9
Fitness effects of CRISPR/Cas9-targeting of long noncoding RNA genes
https://www.nature.com/articles/s41587-020-0428-0
Pervasive head-to-tail insertions of DNA templates mask desired CRISPR-Cas9-mediated genome editing events.
https://advances.sciencemag.org/content/6/7/eaax2941
Multiplexed conditional genome editing with Cas12a in Drosophila
https://www.biorxiv.org/content/10.1101/2020.02.26.966333v1
Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptides.
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-01956-x
Spatial and temporal control of gene manipulation in Drosophila via drug-activated Cas9 nucleases.
https://www.sciencedirect.com/science/article/pii/S0965174820300254
A toxin-antidote CRISPR gene drive system for regional population modification.
https://www.nature.com/articles/s41467-020-14960-3
GO: a functional reporter system to identify and enrich base editing activity
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa124/576...
BIG-TREE: Base-Edited Isogenic hPSC Line Generation Using a Transient Reporter for Editing Enrichment
https://www.sciencedirect.com/science/article/pii/S2213671119304527
A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA.
https://www.nature.com/articles/s41467-020-14977-8
Tandem Paired Nicking Promotes Precise Genome Editing with Scarce Interference by p53
https://www.sciencedirect.com/science/article/pii/S2211124719317218
Multiplex CRISPR/Cas screen in regenerating haploid limbs of chimeric Axolotls.
https://elifesciences.org/articles/48511
A Small Molecule-Controlled Cas9 Repressible System.
https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S21...(20)30006-8
Quality Control Strategy for CRISPR-Cas9-Based Gene Editing Complicated by a Pseudogene.
https://www.frontiersin.org/articles/10.3389/fgene.2019.01297/full
Genome-wide In Vivo CNS Screening Identifies Genes that Modify CNS Neuronal Survival and mHTT Toxicity.
https://linkinghub.elsevier.com/retrieve/pii/S0896-6273(20)30004-0
Efficient and flexible tagging of endogenous genes by homology-independent intron targeting.
https://genome.cshlp.org/content/early/2019/06/25/gr.246413.118?top=1
Cell-cell contact-induced gene editing/activation in mammalian cells using a synNotch-CRISPR/Cas9 system.
https://link.springer.com/article/10.1007/s13238-020-00690-1
Expanding the genome-targeting scope and the site selectivity of high-precision base editors.
https://www.nature.com/articles/s41467-020-14465-z
Identification of functional regulatory elements in the human genome using pooled CRISPR screens.
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6497-0
Multiple Input Sensing and Signal Integration Using a Split Cas12a System
https://www.sciencedirect.com/science/article/pii/S109727652030037X
Genetic screens in isogenic mammalian cell lines without single cell cloning
https://www.nature.com/articles/s41467-020-14620-6
Universal and Naked-Eye Gene Detection Platform Based on CRISPR/Cas12a/13a System.
https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b05597
High-throughput analysis of the activities of xCas9, SpCas9-NG and SpCas9 at matched and mismatched target sequences in human cells.
https://www.nature.com/articles/s41551-019-0505-1
Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses.
https://www.nature.com/articles/s41551-019-0501-5
A transcomplementing gene drive provides a flexible platform for laboratory investigation and potential field deployment.
https://www.nature.com/articles/s41467-019-13977-7
CRISPR/Cas9-mediated precise genome modification by a long ssDNA template in zebrafish.
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6493-4
Single-cell analysis of a mutant library generated using CRISPR-guided deaminase
https://www.biorxiv.org/content/10.1101/610725v2
Generalizable sgRNA design for improved CRISPR/Cas9 editing efficiency.
https://academic.oup.com/bioinformatics/advance-article/doi/10.1093/bioi...
High-throughput screens of PAM-flexible Cas9 variants for gene knock-out and transcriptional modulation
https://www.biorxiv.org/content/10.1101/2020.01.22.916064v1
Production of genetically engineered mice with higher efficiency, lower mosaicism, and multiplexing capability using maternally expressed Cas9.
https://www.nature.com/articles/s41598-020-57996-7
scMAGeCK links genotypes with multiple phenotypes in single-cell CRISPR screens.
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-1928-4
Single AAV-mediated mutation replacement genome editing in limited number of photoreceptors restores vision in mice.
https://www.nature.com/articles/s41467-019-14181-3
Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing.
https://www.nature.com/articles/s41467-020-14362-5
Recording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform.
https://www.nature.com/articles/s41467-019-14012-5
Reactivation of γ-globin expression through Cas9 or base editor to treat β-hemoglobinopathies.
https://www.nature.com/articles/s41422-019-0267-z
RNA isoform screens uncover the essentiality and tumor-suppressor activity of ultraconserved poison exons.
https://www.nature.com/articles/s41588-019-0555-z
A tunable orthogonal coiled-coil interaction toolbox for engineering mammalian cells.
https://www.nature.com/articles/s41589-019-0443-y
Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs.
https://www.nature.com/articles/s41587-019-0387-5
Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors.
https://www.nature.com/articles/s41587-019-0393-7
CRISPR-Cas13d induces efficient mRNA knock-down in animal embryos
https://www.biorxiv.org/content/10.1101/2020.01.13.904763v1
Herpesviral lytic gene functions render the viral genome susceptible to novel editing by CRISPR/Cas9.
https://elifesciences.org/articles/51662
CRISPR-Cas3 induces broad and unidirectional genome editing in human cells.
https://www.nature.com/articles/s41467-019-13226-x
Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency.
https://www.nature.com/articles/s41587-019-0325-6
A bacteriophage nucleus-like compartment shields DNA from CRISPR nucleases.
https://www.nature.com/articles/s41586-019-1786-y
Synthetic chimeric nucleases function for efficient genome editing.
https://www.nature.com/articles/s41467-019-13500-y
Inhibition of histone deacetylase 1 (HDAC1) and HDAC2 enhances CRISPR/Cas9 genome editing.
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz1136/565...
Expanding the editable genome and CRISPR-Cas9 versatility using DNA cutting-free gene targeting based on in trans paired nicking.
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz1121/565...
Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9
https://genome.cshlp.org/content/early/2019/12/12/gr.248559.119.abstract
A bacterial gene-drive system efficiently edits and inactivates a high copy number antibiotic resistance locus
https://www.nature.com/articles/s41467-019-13649-6
Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells
https://www.nature.com/articles/s41592-019-0392-0
Direct capture of CRISPR guides enables scalable, multiplexed, and multi-omic Perturb-seq
https://www.biorxiv.org/content/10.1101/503367v1
Structural basis of DNA targeting by a transposon-encoded CRISPR–Cas system
https://www.nature.com/articles/s41586-019-1849-0
Multi-functional genome-wide CRISPR system for high throughput genotype-phenotype mapping.
https://www.nature.com/articles/s41467-019-13621-4
Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets.
https://www.nature.com/articles/s41467-019-13805-y
Cas12a mediates efficient and precise endogenous gene tagging via MITI: microhomology-dependent targeted integrations.
https://link.springer.com/article/10.1007/s00018-019-03396-8
Allele specific repair of splicing mutations in cystic fibrosis through AsCas12a genome editing.
https://www.nature.com/articles/s41467-019-11454-9
A transient reporter for editing enrichment (TREE) in human cells.
https://academic.oup.com/nar/article/47/19/e120/5552066
Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor.
https://www.nature.com/articles/s41587-019-0331-8
Expanding C-T base editing toolkit with diversified cytidine deaminases.
https://www.nature.com/articles/s41467-019-11562-6
Conditional control of RNA-guided nucleic acid cleavage and gene editing.
https://www.nature.com/articles/s41467-019-13765-3
Assessment of a Split Homing Based Gene Drive for Efficient Knockout of Multiple Genes.
https://www.g3journal.org/content/early/2019/12/27/g3.119.400985
An efficient gene knock-in strategy using 5'-modified double-stranded DNA donors with short homology arms
https://www.nature.com/articles/s41589-019-0432-1
Song et al. AcrIIA5 Inhibits a Broad Range of Cas9 Orthologs by Preventing DNA Target Cleavage. Cell Rep. 2019 Nov 26;29(9):2579-2589.e4.
Cancellieri et al. CRISPRitz: rapid, high-throughput, and variant-aware in silico off-target site identification for CRISPR genome editing. Bioinformatics. 2019 Nov 25. pii: btz867.
Wells et al. Ranking of non-coding pathogenic variants and putative essential regions of the human genome. Nat Commun. 2019 Nov 20;10(1):5241.
Webber et al. Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors. Nat Commun. 2019 Nov 19;10(1):5222.
Wierson et al. Expanding the CRISPR Toolbox with ErCas12a in Zebrafish and Human Cells. CRISPR J. 2019 Nov 19.
Alexander et al. Concurrent genome and epigenome editing by CRISPR-mediated sequence replacement. BMC Biol. 2019 Nov 18;17(1):90.
Askary et al. In situ readout of DNA barcodes and single base edits facilitated by in vitro transcription. Nat Biotechnol. 2019 Nov 18.
Cermon et al. Harnessing type I CRISPR-Cas systems for genome engineering in human cells. Nat Biotechnol. 2019 Nov 18.
Camsund et al. Time-resolved imaging-based CRISPRi screening. Nat Methods. 2019 Nov 18.
Paix et al. Rapid Tagging of Human Proteins with Fluorescent Reporters by Genome Engineering using Double-Stranded DNA Donors. Curr Protoc Mol Biol. 2019 Dec;129(1):e102.
Kulkarni et al. Programmable CRISPR interference for gene silencing using Cas13a in mosquitoes. bioRxiv. Nov. 18, 2019.
Fei et al. Deciphering essential cistromes using genome-wide CRISPR screens. PNAS 2019 Nov 14. pii: 201908155.
Wei et al. CRISPR-based modular assembly of a UAS-cDNA/ORF plasmid library for over 5,500 Drosophila genes conserved in humans. Genome Res. 2019 Nov 13. pii: gr.250811.119.
Canaj et al. Deep profiling reveals substantial heterogeneity of integration outcomes in CRISPR knock-in experiments. bioRxiv. Nov. 13, 2019.
Minev et al. Rapid in vitro production of single-stranded DNA. Nucleic Acids Res. 2019 Nov 12. pii: gkz998.
Garcia et al. Anti-CRISPR AcrIIA5 Potently Inhibits All Cas9 Homologs Used for Genome Editing. Cell Rep. 2019 Nov 12;29(7):1739-1746.e5.
Chiarella et al. Dose-dependent activation of gene expression is achieved using CRISPR and small molecules that recruit endogenous chromatin machinery. Nat Biotechnol. 2019 Nov 11.
Hoshijima et al. Highly Efficient CRISPR-Cas9-Based Methods for Generating Deletion Mutations and F0 Embryos that Lack Gene Function in Zebrafish. Dev Cell. 2019 Nov 7.
Querques et al. A highly soluble Sleeping Beauty transposase improves control of gene insertion. Nat Biotechnol. 2019 Nov 4.
Kanca et al. An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms. Elife. 2019 Nov 1;8. pii: e51539.
Wainberg et al. A genome-wide almanac of co-essential modules assigns function to uncharacterized genes. bioRxiv. Nov. 1, 2019.
Li et al. One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish. Elife. 2019 Oct 30;8. pii: e48081.
Schmidt et al. Nucleic acid cleavage with a hyperthermophilic Cas9 from an uncultured Ignavibacterium. PNAS. 2019 Oct 28. pii: 201904273.
Grajcarek et al. Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations. Nat Commun. 2019 Oct 24;10(1):4856.
Feldman et al. Optical Pooled Screens in Human Cells. Cell. 2019 Oct 17;179(3):787-799.e17.
Wang et al. Multiplexed activation of endogenous genes by CRISPRa elicits potent antitumor immunity. Nat Immunol. 2019 Oct 14.
Watters et al. Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. bioRxiv. Oct. 9, 2019.
Reis et al. Simultaneous repression of multiple bacterial genes using nonrepetitive extra-long sgRNA arrays. Nat Biotechnol. 2019 Oct 7.
Young et al. Genomic and phenotypic analyses of six offspring of a genome-edited hornless bull. Nat Biotechnol. 2019 Oct 7.
He et al. De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens. Nat Commun. 2019 Oct 4;10(1):4541.
Hamilton et al. Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing. Nat Commun. 2019 Oct 4;10(1):4544.
Karageorgi et al. Genome editing retraces the evolution of toxin resistance in the monarch butterfly. Nature. 2019 Oct 2.
Acharya et al. Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing. PNAS. 2019 Sep 30. pii: 201818461.
Tan et al. Rationally engineered Staphylococcus aureus Cas9 nucleases with high genome-wide specificity. PNAS. 2019 Sep 30. pii: 201906843.
Kim et al. Adenine base editors catalyze cytosine conversions in human cells. Nat Biotechnol. 2019 Sep 23.
Taghbalout et al. Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways. Nat Commun. 2019 Sep 20;10(1):4296.
Molina et al. Structure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas. Nat Commun. 2019 Sep 20;10(1):4302.
Macias et al. Cas9-mediated gene-editing in the malaria mosquito Anopheles stephensi by ReMOT Control. bioRxiv. Sept. 19, 2019.
Bhoobalan-Chitty et al. Inhibition of Type III CRISPR-Cas Immunity by an Archaeal Virus-Encoded Anti-CRISPR Protein. Cell. 2019 Sep 19. pii: S0092-8674(19)31009-8.
Wang et al. Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning. Nat Commun. 2019 Sep 19;10(1):4284.
Songailiene et al. Decision-Making in Cascade Complexes Harboring crRNAs of Altered Length. Cell Rep. 2019 Sep 17;28(12):3157-3166.e4.
Whinn et al. Nuclease dead Cas9 is a programmable roadblock for DNA replication. Sci Rep. 2019 Sep 16;9(1):13292.
Lin et al. Off-target toxicity is a common mechanism of action of cancer drugs undergoing clinical trials. Sci Transl Med. 2019 Sep 11;11(509). pii: eaaw8412.
Forsberg et al. Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome. Elife. 2019 Sep 10;8. pii: e46540.
Chen et al. A biodegradable nanocapsule delivers a Cas9 ribonucleoprotein complex for in vivo genome editing. Nat Nanotechnol. 2019 Sep 9.
Tycko et al. Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements. Nat Commun. 2019 Sep 6;10(1):4063.
Tuladhar et al. CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation. Nat Commun. 2019 Sep 6;10(1):4056.
Gomez-Ospina et al. Human genome-edited hematopoietic stem cells phenotypically correct Mucopolysaccharidosis type I. Nat Commun. 2019 Sep 6;10(1):4045.
Grunewald et al. CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep;37(9):1041-1048.
Campa et al. Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts. Nat Methods. 2019 Sep;16(9):887-893.
Nihongaki et al. A split CRISPR-Cpf1 platform for inducible genome editing and gene activation. Nat Chem Biol. 2019 Sep;15(9):882-888.
Liu et al. Programmable RNA N6-methyladenosine editing by CRISPR-Cas9 conjugates. Nat Chem Biol. 2019 Sep;15(9):865-871.
Kweon et al. A CRISPR-based base-editing screen for the functional assessment of BRCA1 variants. Oncogene. 2019 Aug 29.
Fleiss et al. Reshuffling yeast chromosomes with CRISPR/Cas9. PLoS Genet. 2019 Aug 29;15(8):e1008332.
Sanson et al. Optimization of AsCas12a for combinatorial genetic screens in human cells. bioRxiv. Aug. 28, 2019.
Rasys et al. CRISPR-Cas9 Gene Editing in Lizards through Microinjection of Unfertilized Oocytes. Cell Rep. 2019 Aug 27;28(9):2288-2292.e3.
Liu et al. Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria. Nat Commun. 2019 Aug 26;10(1):3693.
Gurumurthy et al. Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation. Genome Biol. 2019 Aug 26;20(1):171.
English et al. Programmable CRISPR-responsive smart materials. Science. 2019 Aug 23;365(6455):780-785.
Norman et al. Exploring genetic interaction manifolds constructed from rich single-cell phenotypes. Science. 2019 Aug 23;365(6455):786-793.
Dong et al. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell. 2019 Aug 22;178(5):1189-1204.e23.
Yosef et al. A genetic system for biasing the sex ratio in mice. EMBO Rep. 2019 Aug;20(8):e48269.
Choi et al. Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9. Nat Methods. 2019 Aug;16(8):722-730.
Keough et al. AlleleAnalyzer: a tool for personalized and allele-specific sgRNA design. Genome Biol. 2019 Aug 15;20(1):167.
Brown et al. CRISPR screens are feasible in TP53 wild‐type cells. Mol Syst Biol. 2019 Aug;15(8):e8679.
Josipovic et al. Antagonistic and synergistic epigenetic modulation using orthologous CRISPR/dCas9-based modular system. Nucleic Acids Res. 2019 Aug 14. pii: gkz709.
Bhatt et al. Targeted DNA transposition in vitro using a dCas9-transposase fusion protein. bioRxiv. April 12, 2019.
Lim et al. A conjugation platform for CRISPR-Cas9 allows efficient β-cell engineering. bioRxiv. August 12, 2019.
Lee et al. Cytosine but not adenine base editor generates mutations in mice. bioRxiv. August 12, 2019.
Knott et al. Structural basis for AcrVA4 inhibition of specific CRISPR-Cas12a. Elife. 2019 Aug 9;8. pii: e49110.
Ratner et al. Catalytically Active Cas9 Mediates Transcriptional Interference to Facilitate Bacterial Virulence. Mol Cell. 2019 Aug 8;75(3):498-510.e5.
Kim et al. CRISPR DNA elements controlling site-specific spacer integration and proper repeat length by a Type II CRISPR-Cas system. Nucleic Acids Res. 2019 Aug 8. pii: gkz677.
Sakata et al. A single CRISPR base editor to induce simultaneous C-to-T and A-to-G mutations. bioRxiv. August 8, 2019.
Riesenberg et al. Simultaneous precise editing of multiple genes in human cells. Nucleic Acids Res. 2019 Aug 8. pii: gkz669. d
Martens et al. Visualisation of dCas9 target search in vivo using an open-microscopy framework. Nat Commun. 2019 Aug 7;10(1):3552.
Liu et al. Establishment of knockout adult sea urchins by using a CRISPR-Cas9 system. Dev Growth Differ. 2019 Aug;61(6):378-388.
Miller et al. Enhancing gene editing specificity by attenuating DNA cleavage kinetics. Nat Biotechnol. 2019 Aug;37(8):945-952.
Garcia-Marques. Unlimited Genetic Switches for Cell-Type-Specific Manipulation. Neuron. 2019 Jul 31. pii: S0896-6273(19)30603-8.
