– – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – –
Orthogonal tuning of gene expression noise using CRISPR-Cas.
https://academic.oup.com/nar/article/48/13/e76/5849902
Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors
https://www.nature.com/articles/s41587-019-0393-7
A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing
https://www.nature.com/articles/s41587-020-0535-y
Dual base editor catalyzes both cytosine and adenine base conversions in human cells
https://www.nature.com/articles/s41587-020-0527-y
A single CRISPR base editor to induce simultaneous C-to-T and A-to-G mutations
https://www.biorxiv.org/content/10.1101/729269v1.abstract
Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response
https://www.sciencedirect.com/science/article/pii/S2211124720307002
Targeted Perturb-seq enables genome-scale genetic screens in single cells.
https://www.nature.com/articles/s41592-020-0837-5
Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs.
https://www.nature.com/articles/s41477-020-0670-y
Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs.
https://www.nature.com/articles/s41467-020-16542-9
CRISPR-TAPE: Protein-Centric CRISPR Guide Design for Targeted Proteome Engineering
https://www.embopress.org/doi/full/10.15252/msb.20209475
Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants
https://www.nature.com/articles/s41587-020-0537-9
Multilayered VBC Score Predicts sgRNAs That Efficiently Generate Loss-Of-Function Alleles
https://www.nature.com/articles/s41592-020-0850-8
Synergistic Gene Editing in Human iPS Cells via Cell Cycle and DNA Repair Modulation
https://www.nature.com/articles/s41467-020-16643-5
Direct-seq: Programmed gRNA Scaffold for Streamlined scRNA-seq in CRISPR Screen
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-02044-w
Massively multiplexed nucleic acid detection with Cas13
https://www.nature.com/articles/s41586-020-2279-8
Very fast CRISPR on demand
https://science.sciencemag.org/content/368/6496/1265.abstract
Development of CRISPR-Cas13a-based antimicrobials capable of sequence-specific killing of target bacteria
https://www.nature.com/articles/s41467-020-16731-6
Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning
https://www.sciencedirect.com/science/article/pii/S0092867420306322
CRISPR artificial splicing factors
https://www.nature.com/articles/s41467-020-16806-4
CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity
https://www.nature.com/articles/s41587-020-0555-7
Single-cell Lineage Tracing by Integrating CRISPR-Cas9 Mutations With Transcriptomic Data
https://www.nature.com/articles/s41467-020-16821-5
Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA
https://advances.sciencemag.org/content/6/25/eaaz4849?utm_source=TrendMD...
Repurposing type I-F CRISPR-Cas system as a transcriptional activation tool in human cells.
https://www.nature.com/articles/s41467-020-16880-8
CRISPR-assisted Detection of RNA-protein Interactions in Living Cells
https://www.nature.com/articles/s41592-020-0866-0
The Histone Chaperone FACT Induces Cas9 Multi-turnover Behavior and Modifies Genome Manipulation in Human Cells
https://www.sciencedirect.com/science/article/pii/S1097276520303993
Precise, Predictable Multi-Nucleotide Deletions in Rice and Wheat Using APOBEC-Cas9
https://www.nature.com/articles/s41587-020-0566-4
Programmable M 6 A Modification of Cellular RNAs With a Cas13-directed Methyltransferase
https://www.nature.com/articles/s41587-020-0572-6
Efficient Gene Editing of Human Long-Term Hematopoietic Stem Cells Validated by Clonal Tracking
https://www.nature.com/articles/s41587-020-0551-y
Small-Molecule Control of Super-Mendelian Inheritance in Gene Drives
https://www.sciencedirect.com/science/article/pii/S2211124720308226
Toward a translationally independent RNA-based synthetic oscillator using deactivated CRISPR-Cas
https://www.biorxiv.org/content/10.1101/2020.05.13.094730v1.abstract
A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity
https://science.sciencemag.org/content/369/6499/54.abstract
Decipher the complexity of cis-regulatory regions by a modified Cas9.
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235530
Upgraded CRISPR/Cas9 Tools for Tissue-Specific Mutagenesis in Drosophila
https://www.biorxiv.org/content/10.1101/2020.07.02.185652v1.abstract
Multilayered VBC score predicts sgRNAs that efficiently generate loss-of-function alleles
https://www.nature.com/articles/s41592-020-0850-8
Sequence-specific Prediction of the Efficiencies of Adenine and Cytosine Base Editors
https://www.nature.com/articles/s41587-020-0573-5
Targeted, efficient sequence insertion and replacement in rice
https://www.nature.com/articles/s41587-020-0581-5
Blackjack Mutations Improve the On-Target Activities of Increased Fidelity Variants of SpCas9 With 5'G-extended sgRNAs
https://www.nature.com/articles/s41467-020-15021-5
A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing
https://www.nature.com/articles/s41586-020-2477-4
A High-Throughput Small Molecule Screen Identifies Farrerol as a Potentiator of CRISPR/Cas9-mediated Genome Editing
https://elifesciences.org/articles/56008
High-fidelity SaCas9 Identified by Directional Screening in Human Cells
https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.30...
In Vivo Repair of a Protein Underlying a Neurological Disorder by Programmable RNA Editing
https://www.biorxiv.org/content/10.1101/2020.02.26.966820v1.abstract
Chromothripsis as an on-target consequence of CRISPR-Cas9 genome editing
https://www.biorxiv.org/content/10.1101/2020.07.13.200998v1.abstract
Optimization of AsCas12a for combinatorial genetic screens in human cells
https://www.nature.com/articles/s41587-020-0600-6
High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening
https://www.nature.com/articles/s41467-020-17209-1
CRISPR-CasΦ from huge phages is a hypercompact genome editor
https://science.sciencemag.org/content/369/6501/333.abstract
Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA
https://www.nature.com/articles/s41467-020-17411-1
Bi-FoRe: an efficient bidirectional knockin strategy to generate pairwise conditional alleles with fluorescent indicators
https://link.springer.com/article/10.1007/s13238-020-00747-1
Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment
https://www.nature.com/articles/s41467-020-17418-8
Enhancement of target specificity of CRISPR-Cas12a by using a chimeric DNA-RNA guide
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa605/587...
CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells
https://www.nature.com/articles/s41587-020-0609-x
New base editors change C to A in bacteria and C to G in mammalian cells
https://www.researchgate.net/publication/343089654_New_base_editors_chan...
Bidirectional titration of yeast gene expression using a pooled CRISPR guide RNA approach
https://www.pnas.org/content/117/31/18424.short
A programmable sequence of reporters for lineage analysis
https://www.nature.com/articles/s41593-020-0676-9
Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters
https://www.nature.com/articles/s41587-020-0614-0
DNA capture by a CRISPR-Cas9–guided adenine base editor
https://science.sciencemag.org/content/369/6503/566.abstract
CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos
https://www.biorxiv.org/content/10.1101/2020.01.13.904763v1.abstract
Engineering designer beta cells with a CRISPR-Cas9 conjugation platform
https://www.nature.com/articles/s41467-020-17725-0
In vitro Cas9-assisted editing of modular polyketide synthase genes to produce desired natural product derivatives
https://www.nature.com/articles/s41467-020-17769-2
Scarless engineering of the Drosophila genome near any site-specific integration site
https://www.biorxiv.org/content/10.1101/2020.08.13.249656v1.abstract
A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
https://www.nature.com/articles/s41467-020-17962-3
An in vivo KRAS allelic series reveals distinct phenotypes of common oncogenic variants
https://cancerdiscovery.aacrjournals.org/content/early/2020/07/24/2159-8...
CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs.
https://www.nature.com/articles/s41467-020-17952-5
Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs
https://www.nature.com/articles/s41587-019-0178-z
Controlled Cycling and Quiescence Enables Efficient HDR in Engraftment-Enriched Adult Hematopoietic Stem and Progenitor Cells
https://www.sciencedirect.com/science/article/pii/S2211124720310822
CRISPR-nRAGE, a Cas9 nickase-reverse transcriptase assisted versatile genetic engineering toolkit for E. coli
https://www.biorxiv.org/content/10.1101/2020.09.02.279141v1
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos
https://www.nature.com/articles/s41598-020-71412-0
Synthetic immunomodulation with a CRISPR super-repressor in vivo
https://www.nature.com/articles/s41556-020-0563-3
Background-suppressed live visualization of genomic loci with an improved CRISPR system based on a split fluorophore
https://genome.cshlp.org/content/early/2020/09/04/gr.260018.119.short?rss=1
Engineering multiple species-like genetic incompatibilities in insects
https://www.nature.com/articles/s41467-020-18348-1
Massively parallel kinetic profiling of natural and engineered CRISPR nucleases.
https://www.nature.com/articles/s41587-020-0646-5
Mutation-Independent Allele-Specific Editing by CRISPR-Cas9, a Novel Approach to Treat Autosomal Dominant Disease
https://www.sciencedirect.com/science/article/abs/pii/S1525001620302367
A Redox-Based Electrogenetic CRISPR System to Connect With and Control Biological Information Networks
https://www.nature.com/articles/s41467-020-16249-x Massively parallel assessment of human variants with base editor screens
https://www.biorxiv.org/content/10.1101/2020.05.17.100818v1
Pronuclear Microinjection during S-Phase Increases the Efficiency of CRISPR-Cas9-Assisted Knockin of Large DNA Donors in Mouse Zygotes
https://www.sciencedirect.com/science/article/pii/S2211124720306069
Multiplex enCas12a screens show functional buffering by paralogs is systematically absent from genome-wide CRISPR/Cas9 knockout screens
https://www.biorxiv.org/content/10.1101/2020.05.18.102764v1
A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects.
https://www.nature.com/articles/s41592-020-0832-x
Cas9 Activates the p53 Pathway and Selects for p53-inactivating Mutations
https://www.nature.com/articles/s41588-020-0623-4
A Cas9 With PAM Recognition for Adenine Dinucleotides
https://www.nature.com/articles/s41467-020-16117-8
Genome-wide CRISPR Screening Reveals Genes Essential for Cell Viability and Resistance to Abiotic and Biotic Stresses in Bombyx mori
https://genome.cshlp.org/content/early/2020/05/18/gr.249045.119
Parallel CRISPR-Cas9 screens clarify impacts of p53 on screen performance
https://elifesciences.org/articles/55325
Targeted In Situ Protein Diversification and Intra-organelle Validation in Mammalian Cells
https://www.sciencedirect.com/science/article/pii/S2451945620300684
Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing
https://www.sciencedirect.com/science/article/pii/S2211124720306422
Enhanced Golic+: Highly effective CRISPR gene targeting and transgene HACKing in Drosophila
https://dev.biologists.org/content/early/2020/05/24/dev.181974
Evaluation of Off-Targets Predicted by sgRNA Design Tools
https://www.sciencedirect.com/science/article/pii/S0888754319307086
BEON: A Functional Fluorescence Reporter for Quantification and Enrichment of Adenine Base-Editing Activity
https://www.sciencedirect.com/science/article/abs/pii/S1525001620301908
Targeted mRNA Demethylation Using an Engineered dCas13b-ALKBH5 Fusion Protein
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa269/582...
Analysis and minimization of cellular RNA editing by DNA adenine base editors.
https://advances.sciencemag.org/content/5/5/eaax5717
Massively Parallel CRISPRi Assays Reveal Concealed Thermodynamic Determinants of dCas12a Binding
https://www.pnas.org/content/early/2020/05/05/1918685117
Genetic interaction mapping and exon-resolution functional genomics with a hybrid Cas9–Cas12a platform
https://www.nature.com/articles/s41587-020-0437-z
Fitness effects of CRISPR/Cas9-targeting of long noncoding RNA genes
https://www.nature.com/articles/s41587-020-0428-0
Reply to: Fitness effects of CRISPR/ Cas9-targeting of long noncoding RNA genes
https://www.nature.com/articles/s41587-020-0431-5
Increasing the Efficiency and Targeting Range of Cytidine Base Editors Through Fusion of a Single-Stranded DNA-binding Protein Domain
https://www.nature.com/articles/s41556-020-0518-8
An Engineered ScCas9 With Broad PAM Range and High Specificity and Activity
https://www.nature.com/articles/s41587-020-0517-0
A Male-Biased Sex-Distorter Gene Drive for the Human Malaria Vector Anopheles Gambiae
https://www.nature.com/articles/s41587-020-0508-1
Endogenous CRISPR/Cas9 Arrays for Scalable Whole-Organism Lineage Tracing
https://dev.biologists.org/content/147/9/dev184481
Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope.
https://www.nature.com/articles/s41467-020-15892-8
Plants with genetically encoded autoluminescence
https://www.nature.com/articles/s41587-020-0500-9
Enhancement of homology-directed repair with chromatin donor templates in cells
https://elifesciences.org/articles/55780
Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
https://www.nature.com/articles/s41467-020-15845-1
Safety and feasibility of CRISPR-edited T cells in patients with refractory non-small-cell lung cancer
https://www.nature.com/articles/s41591-020-0840-5
CRISPR-Cas13 Inhibitors Block RNA Editing in Bacteria and Mammalian Cells
https://www.sciencedirect.com/science/article/abs/pii/S1097276520302252
Massively Multiplexed Nucleic Acid Detection Using Cas13
https://www.nature.com/articles/s41586-020-2279-8
CRISPR/Cas9 mediated genetic resource for unknown kinase and phosphatase genes in Drosophila
https://www.nature.com/articles/s41598-020-64253-4
ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons
https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.30...
Perturbing Proteomes at Single Residue Resolution Using Base Editing
https://www.nature.com/articles/s41467-020-15796-7
Systematic in Vitro Profiling of Off-Target Affinity, Cleavage and Efficiency for CRISPR Enzymes
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa231/582...
Enabling Large-Scale Genome Editing at Repetitive Elements by Reducing DNA Nicking
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa239/582...
Multiplex secretome engineering enhances recombinant protein production and purity
https://www.nature.com/articles/s41467-020-15866-w
Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice
https://www.sciencedirect.com/science/article/pii/S0092867420302865
Extinction of All Infectious HIV in Cell Culture by the CRISPR-Cas12a System With Only a Single crRNA
https://academic.oup.com/nar/article/doi/10.1093/nar/gkaa226/5819595
Rewiring of Endogenous Signaling Pathways to Genomic Targets for Therapeutic Cell Reprogramming
https://www.nature.com/articles/s41467-020-14397-8
Directed evolution of adenine base editors with increased activity and therapeutic application.
https://www.nature.com/articles/s41587-020-0491-6
Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies
https://www.cell.com/cell/fulltext/S0092-8674(20)30332-9?dgcid=raven_jbs_aip_email
Combinatorial Single-Cell CRISPR Screens by Direct Guide RNA Capture and Targeted Sequencing
https://www.nature.com/articles/s41587-020-0470-y
Quantification of the affinities of CRISPR-Cas9 nucleases for cognate protospacer adjacent motif (PAM) sequences.
https://www.jbc.org/content/early/2020/04/01/jbc.RA119.012239.abstract
CRISPR Screens in Cancer Spheroids Identify 3D Growth-Specific Vulnerabilities
https://www.nature.com/articles/s41586-020-2099-x
Therapeutic base editing of human hematopoietic stem cells
https://www.nature.com/articles/s41591-020-0790-y
Genetic Interaction Mapping and Exon-Resolution Functional Genomics With a Hybrid Cas9-Cas12a Platform
https://www.nature.com/articles/s41587-020-0437-z
Targeted nanopore sequencing with Cas9-guided adapter ligation
https://www.nature.com/articles/s41587-020-0407-5
Abundance of conserved CRISPR-Cas9 target sites within the highly polymorphic genomes of Anopheles and Aedes mosquitoes.
https://www.nature.com/articles/s41467-020-15204-0
Prime genome editing in rice and wheat
https://www.nature.com/articles/s41587-020-0455-x
Conditional Single Vector CRISPR/SaCas9 Viruses for Efficient Mutagenesis in the Adult Mouse Nervous System
https://www.sciencedirect.com/science/article/pii/S2211124720302667
Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
https://science.sciencemag.org/content/early/2020/03/25/science.aba8853
A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing.
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-01989-2
Cas9-Mediated Gene-Editing in the Malaria Mosquito Anopheles stephensi by ReMOT Control.
https://www.g3journal.org/content/early/2020/03/02/g3.120.401133.long
Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling.
https://www.pnas.org/content/117/11/5853.abstract
Fast and efficient generation of knock-in human organoids using homology-independent CRISPR-Cas9 precision genome editing.
https://www.nature.com/articles/s41556-020-0472-5
Bridge helix arginines play a critical role in Cas9 sensitivity to mismatches.
https://www.nature.com/articles/s41589-020-0490-4
A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA
https://www.nature.com/articles/s41467-020-14977-8
Programmed chromosome fission and fusion enable precise large-scale genome rearrangement and assembly.
https://science.sciencemag.org/content/365/6456/922
RNA-guided retargeting of Sleeping Beauty transposition in human cells.
https://elifesciences.org/articles/53868
OffScan: a universal and fast CRISPR off-target sites detection tool.
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-6241-9
Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
https://www.ncbi.nlm.nih.gov/pubmed/32160537
Capturing RNA-protein interaction via CRUIS.
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa143/578...
CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects.
https://www.jbc.org/content/early/2020/03/11/jbc.RA120.012933.abstract
CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway.
https://link.springer.com/article/10.1007%2Fs13238-020-00699-6
CRISPR-Cas12b enables efficient plant genome engineering.
https://www.nature.com/articles/s41477-020-0614-6
Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
https://www.nature.com/articles/s41467-020-15226-8
Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping
https://www.nature.com/articles/s41467-020-14957-y
Comprehensive In Vivo Interrogation Reveals Phenotypic Impact of Human Enhancer Variants.
https://www.sciencedirect.com/science/article/pii/S0092867420302087
Highly Parallel Profiling of Cas9 Variant Specificity.
https://www.sciencedirect.com/science/article/abs/pii/S109727652030143X
CAMIO: a transgenic CRISPR pipeline to create diverse targeted genome deletions in Drosophila.
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa177/580...
Deep learning improves the ability of sgRNA off-target propensity prediction.
https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-020-...
Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.
https://www.nature.com/articles/s41587-020-0414-6
Targeted nanopore sequencing with Cas9-guided adapter ligation.
https://www.nature.com/articles/s41587-020-0407-5
Continuous evolution of SpCas9 variants compatible with non-G PAMs.
https://www.nature.com/articles/s41587-020-0412-8
Synthetic CRISPR/Cas9 reagents facilitate genome editing and homology directed repair.
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa085/573...
CRISPR-Based Adenine Editors Correct Nonsense Mutations in a Cystic Fibrosis Organoid Biobank.
https://www.sciencedirect.com/science/article/pii/S1934590920300199
Multiplexed and tunable transcriptional activation by promoter insertion using nuclease-assisted vector integration.
https://academic.oup.com/nar/article/47/12/e67/5424073
MagnEdit-interacting factors that recruit DNA-editing enzymes to single base targets.
https://www.life-science-alliance.org/content/3/4/e201900606
SpCas9-NG self-targets the sgRNA sequence in plant genome editing.
https://www.nature.com/articles/s41477-020-0603-9
Fitness effects of CRISPR/Cas9-targeting of long noncoding RNA genes
https://www.nature.com/articles/s41587-020-0428-0
Pervasive head-to-tail insertions of DNA templates mask desired CRISPR-Cas9-mediated genome editing events.
https://advances.sciencemag.org/content/6/7/eaax2941
Multiplexed conditional genome editing with Cas12a in Drosophila
https://www.biorxiv.org/content/10.1101/2020.02.26.966333v1
Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptides.
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-01956-x
Spatial and temporal control of gene manipulation in Drosophila via drug-activated Cas9 nucleases.
https://www.sciencedirect.com/science/article/pii/S0965174820300254
A toxin-antidote CRISPR gene drive system for regional population modification.
https://www.nature.com/articles/s41467-020-14960-3
GO: a functional reporter system to identify and enrich base editing activity
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa124/576...
BIG-TREE: Base-Edited Isogenic hPSC Line Generation Using a Transient Reporter for Editing Enrichment
https://www.sciencedirect.com/science/article/pii/S2213671119304527
A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA.
https://www.nature.com/articles/s41467-020-14977-8
Tandem Paired Nicking Promotes Precise Genome Editing with Scarce Interference by p53
https://www.sciencedirect.com/science/article/pii/S2211124719317218
Multiplex CRISPR/Cas screen in regenerating haploid limbs of chimeric Axolotls.
https://elifesciences.org/articles/48511
A Small Molecule-Controlled Cas9 Repressible System.
https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S21...(20)30006-8
Quality Control Strategy for CRISPR-Cas9-Based Gene Editing Complicated by a Pseudogene.
https://www.frontiersin.org/articles/10.3389/fgene.2019.01297/full
Genome-wide In Vivo CNS Screening Identifies Genes that Modify CNS Neuronal Survival and mHTT Toxicity.
https://linkinghub.elsevier.com/retrieve/pii/S0896-6273(20)30004-0
Efficient and flexible tagging of endogenous genes by homology-independent intron targeting.
https://genome.cshlp.org/content/early/2019/06/25/gr.246413.118?top=1
Cell-cell contact-induced gene editing/activation in mammalian cells using a synNotch-CRISPR/Cas9 system.
https://link.springer.com/article/10.1007/s13238-020-00690-1
Expanding the genome-targeting scope and the site selectivity of high-precision base editors.
https://www.nature.com/articles/s41467-020-14465-z
Identification of functional regulatory elements in the human genome using pooled CRISPR screens.
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6497-0
Multiple Input Sensing and Signal Integration Using a Split Cas12a System
https://www.sciencedirect.com/science/article/pii/S109727652030037X
Genetic screens in isogenic mammalian cell lines without single cell cloning
https://www.nature.com/articles/s41467-020-14620-6
Universal and Naked-Eye Gene Detection Platform Based on CRISPR/Cas12a/13a System.
https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b05597
High-throughput analysis of the activities of xCas9, SpCas9-NG and SpCas9 at matched and mismatched target sequences in human cells.
https://www.nature.com/articles/s41551-019-0505-1
Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses.
https://www.nature.com/articles/s41551-019-0501-5
A transcomplementing gene drive provides a flexible platform for laboratory investigation and potential field deployment.
https://www.nature.com/articles/s41467-019-13977-7
CRISPR/Cas9-mediated precise genome modification by a long ssDNA template in zebrafish.
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6493-4
Single-cell analysis of a mutant library generated using CRISPR-guided deaminase
https://www.biorxiv.org/content/10.1101/610725v2
Generalizable sgRNA design for improved CRISPR/Cas9 editing efficiency.
https://academic.oup.com/bioinformatics/advance-article/doi/10.1093/bioi...
High-throughput screens of PAM-flexible Cas9 variants for gene knock-out and transcriptional modulation
https://www.biorxiv.org/content/10.1101/2020.01.22.916064v1
Production of genetically engineered mice with higher efficiency, lower mosaicism, and multiplexing capability using maternally expressed Cas9.
https://www.nature.com/articles/s41598-020-57996-7
scMAGeCK links genotypes with multiple phenotypes in single-cell CRISPR screens.
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-1928-4
Single AAV-mediated mutation replacement genome editing in limited number of photoreceptors restores vision in mice.
https://www.nature.com/articles/s41467-019-14181-3
Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing.
https://www.nature.com/articles/s41467-020-14362-5
Recording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform.
https://www.nature.com/articles/s41467-019-14012-5
Reactivation of γ-globin expression through Cas9 or base editor to treat β-hemoglobinopathies.
https://www.nature.com/articles/s41422-019-0267-z
RNA isoform screens uncover the essentiality and tumor-suppressor activity of ultraconserved poison exons.
https://www.nature.com/articles/s41588-019-0555-z
A tunable orthogonal coiled-coil interaction toolbox for engineering mammalian cells.
https://www.nature.com/articles/s41589-019-0443-y
Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs.
https://www.nature.com/articles/s41587-019-0387-5
Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors.
https://www.nature.com/articles/s41587-019-0393-7
CRISPR-Cas13d induces efficient mRNA knock-down in animal embryos
https://www.biorxiv.org/content/10.1101/2020.01.13.904763v1
Herpesviral lytic gene functions render the viral genome susceptible to novel editing by CRISPR/Cas9.
https://elifesciences.org/articles/51662
CRISPR-Cas3 induces broad and unidirectional genome editing in human cells.
https://www.nature.com/articles/s41467-019-13226-x
Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency.
https://www.nature.com/articles/s41587-019-0325-6
A bacteriophage nucleus-like compartment shields DNA from CRISPR nucleases.
https://www.nature.com/articles/s41586-019-1786-y
Synthetic chimeric nucleases function for efficient genome editing.
https://www.nature.com/articles/s41467-019-13500-y
Inhibition of histone deacetylase 1 (HDAC1) and HDAC2 enhances CRISPR/Cas9 genome editing.
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz1136/565...
Expanding the editable genome and CRISPR-Cas9 versatility using DNA cutting-free gene targeting based on in trans paired nicking.
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz1121/565...
Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9
https://genome.cshlp.org/content/early/2019/12/12/gr.248559.119.abstract
A bacterial gene-drive system efficiently edits and inactivates a high copy number antibiotic resistance locus
https://www.nature.com/articles/s41467-019-13649-6
Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells
https://www.nature.com/articles/s41592-019-0392-0
Direct capture of CRISPR guides enables scalable, multiplexed, and multi-omic Perturb-seq
https://www.biorxiv.org/content/10.1101/503367v1
Structural basis of DNA targeting by a transposon-encoded CRISPR–Cas system
https://www.nature.com/articles/s41586-019-1849-0
Multi-functional genome-wide CRISPR system for high throughput genotype-phenotype mapping.
https://www.nature.com/articles/s41467-019-13621-4
Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets.
https://www.nature.com/articles/s41467-019-13805-y
Cas12a mediates efficient and precise endogenous gene tagging via MITI: microhomology-dependent targeted integrations.
https://link.springer.com/article/10.1007/s00018-019-03396-8
Allele specific repair of splicing mutations in cystic fibrosis through AsCas12a genome editing.
https://www.nature.com/articles/s41467-019-11454-9
A transient reporter for editing enrichment (TREE) in human cells.
https://academic.oup.com/nar/article/47/19/e120/5552066
Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor.
https://www.nature.com/articles/s41587-019-0331-8
Expanding C-T base editing toolkit with diversified cytidine deaminases.
https://www.nature.com/articles/s41467-019-11562-6
Conditional control of RNA-guided nucleic acid cleavage and gene editing.
https://www.nature.com/articles/s41467-019-13765-3
Assessment of a Split Homing Based Gene Drive for Efficient Knockout of Multiple Genes.
https://www.g3journal.org/content/early/2019/12/27/g3.119.400985
An efficient gene knock-in strategy using 5'-modified double-stranded DNA donors with short homology arms
https://www.nature.com/articles/s41589-019-0432-1
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