GE Journal Club (genome engineering/gene editing)

CURRENT SCHEDULE

DATE PRESENTERS PAPERS
03/18/2019 NO MEETING – – – – – – – –
03/25/2019 Kendell Clement
Afroditi Petsakou
1) Clement K, et al. CRISPResso2 provides accurate and rapid genome editing sequence analysis. Nat Biotechnol. 2019 Feb 26.
2) TBD
04/01/2019 Pedro Saavedra
Ram Viswanatha
TBD
04/08/2019 Ankita Singh
Arpan Ghosh
TBD
04/15/2019 Baolong Xia
Tracy Zhang
TBD
04/22/2019 NO MEETING – – – – – – – –
04/29/2019 Justin Bosch
Stephanie Mohr
TBD
05/06/2019 NO MEETING – – – – – – – –
05/13/2019 Maria Clara Ingaramo
Ricky Brathwaite
TBD
05/20/2019 Chiao-Lin Chen
Joel Sohn
TBD
05/27/2019 NO MEETING – – – – – – – –

PAST MEETINGS

DATE PRESENTERS PAPERS
03/11/2019 Benjamin Kleinstiver
Henrique Camara
1) Kleinstiver BP, et al. Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11.   
2) Wang XW, et al. 
A microRNA-inducible CRISPR-Cas9 platform serves as a microRNA sensor and cell-type-specific genome regulation tool. Nat Cell Biol. 2019 Feb 25.
03/04/2019   NO MEETING – – – – – – – –
02/25/2019 NO MEETING – – – – – – – –
02/18/2019 NO MEETING – – – – – – – –
02/11/2019 Julio Sainz de Aja
Sarah Bowling
1) Grunwald HA, et al. Super-Mendelian inheritance mediated by CRISPR-Cas9 in the female mouse germline. Nature. 2019 Jan 23.
2) Salvador-Martinez I, et al. 
Is it possible to reconstruct an accurate cell lineage using CRISPR recorders? Elife. 2019 Jan 28;8. pii: e40292.
02/04/2019 NO MEETING – – – – – – – –
01/28/2019 Ah-Ram Kim
Enzo Mameli

1) Matharu N, et al. CRISPR-mediated activation of a promoter or enhancer rescues obesity caused by haploinsufficiency. Science. 2019 Jan 18;363(6424). pii: eaau0629.
2) Oakes BL, et al.
CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification. Cell. 2019 Jan 10;176(1-2):254-267.e16

  NO MEETING 2018: 12/17, 12/24, 12/31
2019: 01/07, 01/14, 01/21
12/10/2018 Henrique Camara
Maria Clara Ingaramo
1) Garcia-Marques J, et al. Unlimited genetic switches for cell-type specific manipulation. bioRxiv [Internet]. Nov. 14, 2018.
2) Farzadfard F, et al.
Single-Nucleotide-Resolution Computing and Memory in Living Cells. bioRxiv [Internet]. Feb. 16, 2018.
12/03/2018 Stephanie Mohr
Justin Bosch
1) Chong Z-S, et al. Pooled extracellular receptor-ligand interaction screening using CRISPR activation. Genome Biol. 2018 Nov 26;19(1):205. 
2) Fueller J, et al.
CRISPR/Cas12a-assisted PCR tagging of mammalian genes. bioRxiv [Internet]. Nov. 20, 2018.
11/26/2018 Baolong Xia
Tracy Zhang
1) Sanson KR, et al. Up, down, and out: optimized libraries for CRISPRa, CRISPRi, and CRISPR-knockout genetic screens. bioRxiv [Internet]. July 2, 2018.
2) Shen MW, et al.
Predictable and precise template-free CRISPR editing of pathogenic variants. Nature. 2018 Nov 7. 
11/19/2018 Johana Vásquez
Chiao-Lin Chen

1) Haney MS, et al. Identification of phagocytosis regulators using magnetic genome-wide CRISPR screens. Nat Genet. 2018 Nov 5. 
2) Harrington LB, et al. 
Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science 18 Oct 2018: eaav4294.

11/12/2018 NO MEETING – – – – – – – –
11/05/2018 Pedro Saavedra
Ram Viswanatha

1) Wroblewska A, et al. Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens. Cell. 2018 Oct 18.
2) Nishimasu H, et al.
Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science. 2018 Aug 30.

10/29/2018 Afroditi Petsakou
Ben Ewen-Campen

1) Schmidt F, et al. Transcriptional recording by CRISPR spacer acquisition from RNA. Nature. 2018 Oct 3.
2) Zafra MP, et al. 
Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Oct;36(9):888-893. 

10/22/2018 Justin Bosch
Stephanie Mohr

1) Akcakaya P, et al. In vivo CRISPR editing with no detectable genome-wide off-target mutations. Nature. 2018 Sep 12.
2) Findlay GM, et al.
Accurate classification of BRCA1 variants with saturation genome editing. Nature. 2018 Sep 12.

10/15/2018

Baolong Xia
Charles Xu

1) Sharon E,  et al. Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing. Cell. 2018 Sep 18. pii: S0092-8674(18)31118-8.
2) Alemany A, et al.
Whole-organism clone tracing using single-cell sequencing. Nature. 2018 Apr 5;556(7699):108-112.

10/08/2018 NO MEETING – – – – – – – –
10/01/2018

Ankita Singh
Chiao-Lin Chen

1) Swings T, et al. CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing. Nat Commun. 2018 Jun 8;9(1):2231.
2)  Allen FR, et al. 
Mutations generated by repair of Cas9-induced double strand breaks are predictable from surrounding sequence. bioRxiv [Internet]. Aug. 25, 2018.

  NO MEETING 09/04, 09/10, 09/17, 09/24

08/27/2018

Pedro Saavedra
Ram Viswanatha

1) Horlbeck MA, et al. Mapping the Genetic Landscape of Human Cells. Cell. 2018 Aug 9;174(4):953-967.e22. 
2) Roche PJR, et al. 
Homology Directed Repair by Cas9:Donor Co-localization in Mammalian Cells. bioRxiv [Internet]. Aug. 6, 2018. 

08/20/2018

Afroditi Petsakou
Ben Ewen-Campen

1) Kundert K, et al. Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs. bioRxiv [Internet]. May 15, 2018.
2) Chaverra-Rodriguez D, et al. 
Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing. Nat Commun. 2018 Aug 1;9(1):3008. 

08/06/2018 Justin Bosch 1) Kosicki M, et al. Repair of double-strand breaks induced by CRISPR-Cas9 leads to large deletions and complex rearrangements. Nat Biotechnol. 2018;36 (8) :765-771.

ABOUT GE JOURNAL CLUB


Who are we?
Researchers at HMS (Post-docs, grad students, technicians, PIs, etc).

What do we talk about?
Genome engineering/gene editing – novel methods, technical issues (e.g. off-target analysis), ethical concerns, etc.

Why are we doing this?
To stay up to date on recent techniques, stimulate critical discussions, establish contacts for collaboration

When/where?
12:30 PM – 1:30 PM Mondays, Room 354 New Research Building (see schedule) 

What’s the format?
Presenters choose what they present. 2x presentations per meeting (30min each). Usually, this is a single recent paper in the field, presented as a PowerPoint to show the figure panels. Presenters can present any topic/format they wish, such as multiple papers, reviews, general discussion, chalk talk, etc.

Do I have to read the paper?
No, but it’s recommended.

Will I have to present?
We hope you will! But this is not strictly required.

I’m scheduled to present, what do I do?
Find a paper/topic you are excited about! The topic can be related to your expertise, or not. We provide a list of suggested papers (below) but feel free to consider alternatives. *Send your paper/topic selection to the organizer by the Friday before your presentation.* When you present, 1) introduce yourself, 2) explain why you selected the paper/topic, and 3) give background on the topic. Feel free to cut out panels or entire figures if it improves the presentation. Keep your presentation aimed at a general audience - most people do not read the paper and some will not be familiar with your topic.

CONTACT

Questions? Want to join the mailing list? Want to present? Have paper suggestions? – Contact Justin Bosch (jabosch@hms.harvard.edu) (Perrimon Lab)

HMS Email Accounts: Click here to subscribe yourself to the mailing list.

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SUGGESTED PAPERS (reverse chronological order)

Kelliher T, et al. One-step genome editing of elite crop germplasm during haploid induction. Nat Biotechnol. 2019 Mar;37(3):287-292.

Labun K, et al. Accurate analysis of genuine CRISPR editing events with ampliCan. Genome Res. 2019 Mar 8. pii: gr.244293.118.

Cullot G, et al. CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations. Nat Commun. 2019 Mar 8;10(1):1136.

Wegner M, et al. Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome. Elife. 2019 Mar 6;8. pii: e42549.

Min YL, et al. CRISPR-Cas9 corrects Duchenne muscular dystrophy exon 44 deletion mutations in mice and human cells. Sci Adv. 2019 Mar 6;5(3):eaav4324.

Zhang Y, et al. A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae. Nat Commun. 2019 Mar 5;10(1):1053.

Zhu Y, et al. Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins. Mol Cell. 2019 Mar 5. pii: S1097-2765(19)30058-9.

Kim D, et al. Genome-wide target specificity of CRISPR RNA-guided adenine base editors. Nat Biotechnol. 2019 Mar 4. [Epub ahead of print]

Fu BXH, et al. Target-dependent nickase activities of the CRISPR-Cas nucleases Cpf1 and Cas9. Nat Microbiol. 2019 Mar 4. [Epub ahead of print]

Tsui CK, et al. Systematic identification of regulators of antibody-drug conjugate toxicity using CRISPR-Cas9 screens. bioRxiv. Mar. 3, 2019.

Zuo E, et al. Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos. Science. 2019 Feb 28. pii: eaav9973.


Jin S, et al. Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice. Science. 2019 Feb 28. pii: eaaw7166.


O’Brien AR, et al. Unlocking HDR-mediated nucleotide editing by identifying high-efficiency target sites using machine learning. Sci Rep. 2019 Feb 26;9(1):2788.


Newton MD, et al. DNA stretching induces Cas9 off-target activity. Nat Struct Mol Biol. 2019 Feb 25.


Edracki A, et al. A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome Editing. Mol Cell. 2019 Feb 21;73(4):714-726.e4.


Wright AV, et al. A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System. Mol Cell. 2019 Feb 21;73(4):727-737.e3.  


Vicente MM, et al. A CyclinB2-Cas9 fusion promotes the homology-directed repair of double-strand breaks. bioRxiv. Feb. 20, 2019.


Schmidt ST, et al. Nucleic Acid Cleavage with a Hyperthermophilic Cas9 from an Unculturable Ignavibacterium. bioRxiv. Feb. 20, 2019.


Michaels YS, et al. Precise tuning of gene expression levels in mammalian cells. Nat Commun. 2019 Feb 18;10(1):818.


Prowse TA, et al. A Y-chromosome shredding gene drive for controlling pest vertebrate populations. Elife. 2019 Feb 15;8. pii: e41873.


Oberhofer G, et al. Cleave and Rescue, a novel selfish genetic element and general strategy for gene drive. PNAS. 2019 Feb 13. pii: 201816928.


Papikian A, et al. Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun. 2019 Feb 13;10(1):729.


Back S, et al. Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats. Neuron. 2019 Feb 8. pii: S0896-6273(19)30062-5

Katrekar D, et al. In vivo RNA editing of point mutations via RNA-guided adenosine deaminases. Nat Methods. 2019 Feb 8.

Teng F, et al. Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds. Genome Biol. 2019 Feb 5;20(1):15.

Goncalves E, et al. Structural rearrangements generate cell-specific, gene-independent CRISPR-Cas9 loss of fitness effects. Genome Biol. 2019 Feb 5;20(1):27.  

Liu JJ, et al. CasX enzymes comprise a distinct family of RNA-guided genome editors. Nature. 2019 Feb 4.

Ma D, et al. Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information. Nat Comm. 2019 Feb 4;10(1):560.

Graf R, et al. sgRNA Sequence Motifs Blocking Efficient CRISPR/Cas9-Mediated Gene Editing. Cell Rep. 2019 Jan 29;26(5):1098-1103.e3.

Merkle T, et al. Precise RNA editing by recruiting endogenous ADARs with antisense oligonucleotides. Nat Biotech. 2019 Jan 28.

Charlesworth CT, el al. Identification of preexisting adaptive immunity to Cas9 proteins in humans. Nat Med. 2019 Jan 28.

Anderson KM, et al. Targeted Cleavage and Polyadenylation of RNA by CRISPR-Cas13. bioRxiv. Jan. 26, 2019.

Fulco CP, et al. Activity-by-Contact model of enhancer specificity from thousands of CRISPR perturbations. bioRxiv. Jan. 26, 2019.

Fortin JP, et al. Multiple-gene targeting and mismatch tolerance can confound analysis of genome-wide pooled CRISPR screens. Genome Biol. 2019 Jan 25;20(1):21.

Tan J, et al. Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Comm. 2019 Jan 25;10(1):439.

Zhu S, et al. Guide RNAs with embedded barcodes boost CRISPR-pooled screens. Genome Biol. 2019 Jan 24;20(1):20.

Strecker J, et al. Engineering of CRISPR-Cas12b for human genome editing. Nat Commun. 2019 Jan 22;10(1):212. 

Guo M, et al. Structural insights into a high fidelity variant of SpCas9. Cell Res. 2019 Jan 21. 

Tycko J, et al. Identification and mitigation of pervasive off-target activity in CRISPR-Cas9 screens for essential non-coding elements. bioRxiv . Jan. 18, 2019.

Nobles CL, et al. iGUIDE: an improved pipeline for analyzing CRISPR cleavage specificity. Genome Biol. 2019 Jan 17;20(1):14.

Nakamura M, et al. Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells. Nat Comm. 2019 Jan 14;10(1):194.

Rubin AJ, et al. Coupled Single-Cell CRISPR Screening and Epigenomic Profiling Reveals Causal Gene Regulatory Networks. Cell. 2019 Jan 10;176(1-2):361-376.e17.

Gasperini M, et al. A Genome-wide Framework for Mapping Gene Regulation via Cellular Genetic Screens. Cell. 2019 Jan 10;176(1-2):377-390.e19.

Kandul NP, et al. Transforming insect population control with precision guided sterile males with demonstration in flies. Nat Comm. 2019 Jan 8;10(1):84.

Goudarzi M, et al. Individual long non-coding RNAs have no overt functions in zebrafish embryogenesis, viability and fertility. Elife. 2019 Jan 8;8. pii: e40815.

Yan WX, et al. Functionally diverse type V CRISPR-Cas systems. Science. 2019 Jan 4;363(6422):88-91.

Gilles AF, et al. Clonal analysis by tunable CRISPR-mediated excision. Develop. 2019 Jan 4;146(1).

Mangeot PE, et al. Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins. Nat Comm. 2019 Jan 3;10(1):45.

Breinig M, et al. Multiplexed orthogonal genome editing and transcriptional activation by Cas12a. Nat Methods. 2019 Jan;16(1):51-54.

Laughery MF, et al. R-loop formation by dCas9 is mutagenic in Saccharomyces cerevisiae. Nucleic Acids Res. 2018 Dec 21.

Reploge JM, et al. Direct capture of CRISPR guides enables scalable, multiplexed, and multi-omic Perturb-seq. bioRxiv. Dec. 21, 2018.

Globyte V. CRISPR/Cas9 searches for a protospacer adjacent motif by lateral diffusion. EMBO J. 2018 Dec 20. pii: e99466.

Jiang F, et al. Temperature-Responsive Competitive Inhibition of CRISPR-Cas9. Mol Cell. 2018 Dec 19. pii: S1097-2765(18)30986-9.

Li B, et al. Synthetic Oligonucleotides Inhibit CRISPR-Cpf1-Mediated Genome Editing. Cell Rep. 2018 Dec 18;25(12):3262-3272.e3.

Chakrabarti AM, et al. Target-Specific Precision of CRISPR-Mediated Genome Editing. Mol Cell. 2018 Dec 10. pii: S1097-2765(18)31001-3.

Siu KH, et al. Riboregulated toehold-gated gRNA for programmable CRISPR–Cas9 function. Nat Chem Biol. 2018 Dec 10.

Yan WX, et al. Functionally diverse type V CRISPR-Cas systems. Science. 2018 Dec 6. eaav7271

Amrani N, et al. NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome Biol. 2018 Dec 5;19(1):214.

Yang S, et al. Shortening the Half-Life of Cas9 Maintains Its Gene Editing Ability and Reduces Neuronal Toxicity. Cell Rep. 2018 Dec 04. 25:10, P2653-2659.E3.

Poe et al. Robust CRISPR/Cas9-mediated tissue specific mutagenesis reveals gene redundancy and perdurance in Drosophila. Genetics. 2018 Nov 30.

Ikeda K, et al. Efficient scarless genome editing in human pluripotent stem cells. Nat Methods. 2018 Nov 30. 

Chen B, et al. Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag. Nat Comm. 2018 Nov 29;9(1):5065.

Chen W, et al. Massively parallel profiling and predictive modeling of the outcomes of CRISPR/Cas9-mediated double-strand break repair. bioRxiv. Nov. 28, 2018.

Allen F, et al. Predicting the mutations generated by repair of Cas9-induced double-strand breaks. Nat Biotech. 2018 Nov 27. 

Hoffmann MD, et al. Cell-specific CRISPR/Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins. bioRxiv. Nov. 27, 2018.

Yang Y, et al. Base editing generates substantial off-target single nucleotide variants. bioRxiv. Nov. 27, 2018.

Gao Z, et al. Improvement of the CRISPR-Cpf1 system with ribozyme-processed crRNA. RNA Biol. 2018 Nov 23.

Buchmuller BC, et al. Pooled clone collections by multiplexed CRISPR/Cas12a-assisted gene tagging in yeast. bioRxiv. Nov. 22, 2018.

Bolukbasi MF, et al. Orthogonal Cas9-Cas9 chimeras provide a versatile platform for genome editing. Nat Comm. 2018 Nov 19;9(1):4856. 

Lee HK, et al. Targeting fidelity of adenine and cytosine base editors in mouse embryos. Nat Comm. 2018 Nov 15;9(1):4804.

Burg L, et al. Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish. PLoS Genet. 2018 Nov 14;14(11):e1007754.

Wienert B, et al. Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq. bioRxiv. Nov. 14, 2018.

Zhu H, et al. Spatial control of in vivo CRISPR–Cas9 genome editing via nanomagnets. Nature Biomed Engineer. 2018 Nov 12.

Kim D, et al. DIG-seq: a genome-wide CRISPR off-target profiling method using chromatin DNA. Genome Res. 2018 Nov 9. 

Liu Y, et al. Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotech. 2018 Nov 5. 

Wang X, et al. Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotech. 2018 Nov;36(10):946-949. 

Ma H, et al. CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging. Nat Methods. 2018 Oct 30.

Bubeck F, et al. Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods. 2018 Oct 30.

Chatterjee P, et al. Minimal PAM specificity of a highly similar SpCas9 ortholog. Science Advances  24 Oct 2018: Vol. 4, no. 10, eaau0766.

Liu DL, et al. Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23. 25:4, P884-892.E3.

Guo T, et al. Harnessing accurate non-homologous end joining for efficient precise deletion in CRISPR/Cas9-mediated genome editing. Genome Biol. 2018 Oct 19;19(1):170. 

Wang D, et al. Cas9-mediated allelic exchange repairs compound heterozygous recessive mutations in mice. Nat Biotech. 2018 Oct;36(9):839-842. 

Koblan LW, et al. Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotech. 2018 Oct;36(9):843-846. 

Ting PY, et al. Guide Swap enables genome-scale pooled CRISPR-Cas9 screening in human primary cells. Nat Methods. 2018 Oct 8. 

Wang H, et al. CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear Organization. Cell. 2018 Oct 8. pii: S0092-8674(18)31185-1. 

Shariati A, et al. Reversible disruption of specific transcription factor-DNA interactions using CRISPR/Cas9. bioRxiv. Sept. 28, 2018.

Jakimo N, et al. A Cas9 with Complete PAM Recognition for Adenine Dinucleotides. bioRxiv. Sept. 27, 2018.

Kyrou K, et al. A CRISPR-Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes. Nat Biotech. 2018 Sep 24.

Yuan J, et al. Genetic Modulation of RNA Splicing with a CRISPR-Guided Cytidine Deaminase. Mol Cell. 2018 Sep 21. pii: S1097-2765(18)30741-X.

Oberhofer G, et al. Behavior of homing endonuclease gene drives targeting genes required for viability or female fertility with multiplexed guide RNAs. PNAS. 2018 Sep 17.

Wang H, et al. An Efficient Genome Editing Strategy To Generate Putative Null Mutants in Caenorhabditis elegans Using CRISPR/Cas9. G3. 2018 Sep 17.

Serebrenik YV, et al. Scalable tagging of endogenous genes by homology-independent intron targeting. bioRxiv. Sept. 10, 2018.

Yarrington RM, et al. Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo. PNAS. 2018 Sep 10. 

Champer J, et al. Molecular safeguarding of CRISPR gene drive experiments. bioRxiv. Sept 8, 2018.

Bin Moon S, et al. Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3'-overhang. Nat Comm. 2018 Sep 7;9(1):3651. 

Marino ND, et al. Discovery of widespread Type I and Type V CRISPR-Cas inhibitors. Science. 2018 Sep 6. 

Watters KE, et al. Systematic discovery of natural CRISPR-Cas12a inhibitors. Science. 2018 Sep 6. 

Sinha S, et al. A systematic genome-wide mapping of the oncogenic risks associated with CRISPR-Cas9 editing. bioRxiv. Sept. 3, 2018.

Lee H, et al. Unexpected CRISPR on-target effects. Nat Biotech. 2018 Sep;36(8):703-704. 

Kalhor R, et al. Developmental barcoding of whole mouse via homing CRISPR. Science. 2018 Aug 31;361(6405).

Gutierrez-Triana JA, et al. Efficient single-copy HDR by 5' modified long dsDNA donors. Elife. 2018 Aug 29;7.

Schaefer M, et al. PAVOOC: Designing CRISPR sgRNAs using 3D protein structures and functional domain annotation. bioRxiv. Aug. 23, 2018.

Park HM, et al. Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo. Nat Comm. 2018 Aug 17;9(1):3313.

Gapinske M, et al. CRISPR-SKIP: programmable gene splicing with single base editors. Genome Biol. 2018 Aug 15;19(1):107. 

Sternberg PW, et al. An efficient genome editing strategy to generate putative null mutants in Caenorhabditis elegans using CRISPR/Cas9. bioRxiv. Aug. 13, 2018.

Chakrabarti AM, et al. Target-specific precision of CRISPR-mediated genome editing. bioRxiv. Aug. 9, 2018.

Su K-C, et al. CRISPR/Cas9-based gene targeting using synthetic guide RNAs enables robust cell biological analyses. Mol Biol Cell. 2018 Aug 9:mbcE18040214. 

Kuscu C, et al. Temporal and Spatial Epigenome Editing Allows Precise Gene Regulation in Mammalian Cells. J Mol Biol. 2018 Aug 9. 

Roche PJR, et al. Homology Directed Repair by Cas9:Donor Co-localization in Mammalian Cells. bioRxiv . Aug. 6, 2018.

Lee JK, et al. Directed evolution of CRISPR-Cas9 to increase its specificity. Nat Commun. 2018 Aug 6;9(1):3048. 

Richardson CD, et al. CRISPR-Cas9 genome editing in human cells occurs via the Fanconi anemia pathway. Nat Genet. 2018 Aug;50(8):1132-1139. 

Halperin SO, et al. CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window. Nature. 2018 Aug;560(7717):248-252.

Yeo NC, et al. An enhanced CRISPR repressor for targeted mammalian gene regulation. Nat Methods. 2018 Aug;15(8):611-616. 

Chaverra-Rodriguez D, et al. Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing. Nat Comm. 2018 Aug 1;9(1):3008. 

Gehrke JM, et al. An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities. Nat Biotech. 2018 Jul 30. 

Kandul NP, et al. Transforming Insect Population Control with Precision Guided Sterile Males. bioRxiv. July 26, 2018.

Grunwald HA, et al. Super-Mendelian inheritance mediated by CRISPR/Cas9 in the female mouse germline. bioRxiv. July 7, 2018.

Suzuki T, et al. Switchable genome editing via genetic code expansion. Sci Rep. 2018 Jul 3;8(1):10051.

Ihry RJ, et al. p53 inhibits CRISPR-Cas9 engineering in human pluripotent stem cells. Nat Med. 2018 Jul;24(7):939-946. 

Mello C, et al. Robust genome editing with short single-stranded and long, partially single-stranded DNA donors in C. elegans. bioRxiv. June 20, 2018.

Noble C, et al. Current CRISPR gene drive systems are likely to be highly invasive in wild populations. Elife. 2018 Jun 19;7.

Knapp DJHF, et al. Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression. bioRxiv. June 8, 2018.

Li-Kroeger D, et al. An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila. bioRxiv. June 2, 2018.

Savic N, et al. Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair. Elife. 2018 May 29;7.

Kurata M, et al. Highly Multiplexed Genome Engineering Using CRISPR/Cas9 gRNA Arrays. bioRxiv. May 25, 2018.

Kundert K, et al. Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs. bioRxiv. May 15, 2018.

Liao C, et al. One-step assembly of large CRISPR arrays enables multi-functional targeting and reveals constraints on array design. bioRxiv. May 2, 2018.

Chen JS, et al. CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Science. 2018 Apr 27;360(6387):436-439. 

Tang W, et al. Rewritable multi-event analog recording in bacterial and mammalian cells. Science. 2018 Apr 13;360(6385). 

Hu JH, et al. Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Apr 5;556(7699):57-63.

Tsui C, et al. dCas9-targeted locus-specific protein isolation method identifies histone gene regulators. PNAS. 2018 Mar 20;115(12):E2734-E2741

Chen B, et al. CRISPR-Tag: an Efficient DNA Tagging System in Living Cells. bioRxiv. March 12, 2018.

Kim HK, et al. Deep learning improves prediction of CRISPR-Cpf1 guide RNA activity. Nat Biotech. 2018 Mar;36(3):239-241. 

Casini A, et al. A highly specific SpCas9 variant is identified by in vivo screening in yeast. Nat Biotech. 2018 Mar;36(3):265-271. 

Zhou H, et al. In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nat Neurosci. 2018 Mar;21(3):440-446. 

Poe AR, et al. Highly efficient CRISPR/Cas9-mediated tissue specific mutagenesis in Drosophila. bioRxiv. Feb. 20, 2018.

Strutt SC, et al. RNA-dependent RNA targeting by CRISPR-Cas9. Elife. 2018 Jan 5;7.

Moreno-Mateos MA, et al. CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. Nat Comm. 2017 Dec 8;8(1):2024.

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