Precise genome engineering in using prime editing

Citation:

Bosch JA, Birchak G, Perrimon N. Precise genome engineering in using prime editing. Proc Natl Acad Sci U S A. 2021;118 (1).

Date Published:

2021 Jan 05

Abstract:

Precise genome editing is a valuable tool to study gene function in model organisms. Prime editing, a precise editing system developed in mammalian cells, does not require double-strand breaks or donor DNA and has low off-target effects. Here, we applied prime editing for the model organism and developed conditions for optimal editing. By expressing prime editing components in cultured cells or somatic cells of transgenic flies, we precisely introduce premature stop codons in three classical visible marker genes, , , and Furthermore, by restricting editing to germ cells, we demonstrate efficient germ-line transmission of a precise edit in to 36% of progeny. Our results suggest that prime editing is a useful system in to study gene function, such as engineering precise point mutations, deletions, or epitope tags.

Last updated on 01/15/2021