Citation:
Ni J-Q, Markstein M, Binari R, Pfeiffer B, Liu L-P, Villalta C, et al. Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster. Nat Methods. 2008;5 (1) :49-51.
 | 2008_Nat Meth_Ni.pdf | 225 KB |
| Supplement.pdf | 905 KB |
Date Published:
2008 JanAbstract:
The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.
See also: Research Article
