%0 Journal Article %J Nat Methods %D 2008 %T Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster. %A Ni, Jian-Quan %A Markstein, Michele %A Binari, Richard %A Pfeiffer, Barret %A Liu, Lu-Ping %A Villalta, Christians %A Booker, Matthew %A Perkins, Lizabeth %A Perrimon, Norbert %K Animals %K Drosophila melanogaster %K Drosophila Proteins %K Gene Targeting %K Genetic Vectors %K RNA Interference %X

The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.

%B Nat Methods %V 5 %P 49-51 %8 2008 Jan %G eng %N 1 %1 http://www.ncbi.nlm.nih.gov/pubmed/18084299?dopt=Abstract %R 10.1038/nmeth1146