@article {969931,
	title = {miR-263a Regulates ENaC to Maintain Osmotic and Intestinal Stem Cell Homeostasis in Drosophila.},
	journal = {Dev Cell},
	volume = {40},
	number = {1},
	year = {2017},
	month = {2017 Jan 09},
	pages = {23-36},
	abstract = {
	Proper regulation of osmotic balance and response to tissue damage is crucial in maintaining intestinal\&nbsp;stem cell (ISC) homeostasis. We found that Drosophila miR-263a downregulates the expression of epithelial sodium channel (ENaC) subunits in enterocytes (ECs) to maintain osmotic and ISC homeostasis. In the absence of miR-263a, the intraluminal surface of the intestine displays dehydration-like phenotypes, Na(+) levels are increased in ECs, stress pathways are activated in ECs, and ISCs overproliferate. Furthermore, miR-263a mutants have increased bacterial load and expression of antimicrobial peptides. Strikingly, these phenotypes are reminiscent of the pathophysiology of cystic fibrosis (CF) in which loss-of-function mutations in the chloride channel CF transmembrane conductance regulator can elevate the activity of ENaC, suggesting that Drosophila could be used as a model for CF. Finally, we provide evidence that overexpression of miR-183, the human ortholog of miR-263a, can also directly target the expressions of all three subunits of human ENaC.

},
	issn = {1878-1551},
	doi = {10.1016/j.devcel.2016.11.023},
	author = {Kim, Kevin and Hung, Ruei-Jiun and Perrimon, Norbert}
}
