@article {887566,
	title = {Design and Generation of Donor Constructs for Genome Engineering in Drosophila.},
	journal = {Cold Spring Harb Protoc},
	volume = {2016},
	number = {9},
	year = {2016},
	month = {2016},
	pages = {pdb.prot090787},
	abstract = {
	The generation of precise alterations to the genome using CRISPR requires the combination of CRISPR and a donor construct containing homology to the target site. A double-strand break is first generated at the target locus using CRISPR. It is then repaired using the endogenous homologous recombination (HR) pathway. When a donor construct is provided, it can be used as a template for HR repair and can therefore be exploited to introduce alterations in the genomic sequence with single base-pair precision. Here we describe a protocol for the generation of donor constructs using Golden Gate assembly and discuss some key considerations for donor construct design for use in Drosophila.

},
	issn = {1559-6095},
	doi = {10.1101/pdb.prot090787},
	author = {Housden, Benjamin E and Perrimon, Norbert}
}
