@article {843581,
	title = {Autosomal P[ovoD1] dominant female-sterile insertions in Drosophila and their use in generating germ-line chimeras.},
	journal = {Development},
	volume = {119},
	number = {4},
	year = {1993},
	month = {1993 Dec},
	pages = {1359-69},
	abstract = {
	The {\textquoteright}dominant female-sterile{\textquoteright} technique used to generate germ-line mosaics in Drosophila is a powerful tool to determine the tissue specificity (germ line versus somatic) of recessive female-sterile mutations as well as to analyze the maternal effect of recessive zygotic lethal mutations. This technique requires the availability of germ-line-dependent, dominant female-sterile (DFS) mutations that block egg laying but do not affect viability. To date only one X-linked mutation, ovoD1 has been isolated that completely fulfills these criteria. Thus the {\textquoteright}DFS technique{\textquoteright} has been largely limited to the X-chromosome. To extend this technique to the autosomes, we have cloned the ovoD1 mutation into a P-element vector and recovered fully expressed P[ovoD1] insertions on each autosomal arm. We describe the generation of these P[ovoD1] strains as well as demonstrate their use in generating germ-line chimeras. Specifically, we show that the Gap1 gene, which encodes a Drosophila homologue of mammalian GTPase-activating protein, is required in somatic follicle cells for embryonic dorsoventral polarity determination.

},
	keywords = {Animals, Chimera, Cloning, Molecular, Drosophila, Female, Gene Transfer Techniques, Genes, Dominant, Genetic Vectors, In Situ Hybridization, Infertility, Female, Mutagenesis, Insertional, Oocytes, Ovarian Follicle, Phenotype},
	issn = {0950-1991},
	author = {Chou, T B and Noll, E and Perrimon, N}
}
