@article {834371,
	title = {Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays.},
	journal = {Nat Methods},
	volume = {3},
	number = {10},
	year = {2006},
	month = {2006 Oct},
	pages = {833-8},
	abstract = {To evaluate the specificity of long dsRNAs used in high-throughput RNA interference (RNAi) screens performed at the Drosophila RNAi Screening Center (DRSC), we performed a global analysis of their activity in 30 genome-wide screens completed at our facility. Notably, our analysis predicts that dsRNAs containing \&gt; or = 19-nucleotide perfect matches identified in silico to unintended targets may contribute to a significant false positive error rate arising from off-target effects. We confirmed experimentally that such sequences in dsRNAs lead to false positives and to efficient knockdown of a cross-hybridizing transcript, raising a cautionary note about interpreting results based on the use of a single dsRNA per gene. Although a full appreciation of all causes of false positive errors remains to be determined, we suggest simple guidelines to help ensure high-quality information from RNAi high-throughput screens.},
	keywords = {Animals, Drosophila melanogaster, False Positive Reactions, Gene Library, Genetic Testing, RNA Interference, RNA, Double-Stranded, Sensitivity and Specificity},
	issn = {1548-7091},
	doi = {10.1038/nmeth935},
	author = {Kulkarni, Meghana M and Booker, Matthew and Silver, Serena J and Friedman, Adam and Hong, Pengyu and Perrimon, Norbert and Mathey-Prevot, Bernard}
}
