@article {834351,
	title = {Functional genomics reveals genes involved in protein secretion and Golgi organization.},
	journal = {Nature},
	volume = {439},
	number = {7076},
	year = {2006},
	month = {2006 Feb 2},
	pages = {604-7},
	abstract = {Yeast genetics and in vitro biochemical analysis have identified numerous genes involved in protein secretion. As compared with yeast, however, the metazoan secretory pathway is more complex and many mechanisms that regulate organization of the Golgi apparatus remain poorly characterized. We performed a genome-wide RNA-mediated interference screen in a Drosophila cell line to identify genes required for constitutive protein secretion. We then classified the genes on the basis of the effect of their depletion on organization of the Golgi membranes. Here we show that depletion of class A genes redistributes Golgi membranes into the endoplasmic reticulum, depletion of class B genes leads to Golgi fragmentation, depletion of class C genes leads to aggregation of Golgi membranes, and depletion of class D genes causes no obvious change. Of the 20 new gene products characterized so far, several localize to the Golgi membranes and the endoplasmic reticulum.},
	keywords = {Animals, Cell Line, Drosophila, Drosophila Proteins, Endoplasmic Reticulum, Genes, Insect, Genes, Reporter, Genomics, Golgi Apparatus, Horseradish Peroxidase, Intracellular Membranes, Protein Sorting Signals, RNA Interference},
	issn = {1476-4687},
	doi = {10.1038/nature04377},
	author = {Bard, Frederic and Casano, Laetitia and Mallabiabarrena, Arrate and Wallace, Erin and Saito, Kota and Kitayama, Hitoshi and Guizzunti, Gianni and Hu, Yue and Wendler, Franz and Dasgupta, Ramanuj and Perrimon, Norbert and Malhotra, Vivek}
}
