@article {824831,
	title = {The transcriptional diversity of 25 Drosophila cell lines.},
	journal = {Genome Res},
	volume = {21},
	number = {2},
	year = {2011},
	month = {2011 Feb},
	pages = {301-14},
	abstract = {Drosophila melanogaster cell lines are important resources for cell biologists. Here, we catalog the expression of exons, genes, and unannotated transcriptional signals for 25 lines. Unannotated transcription is substantial (typically 19\% of euchromatic signal). Conservatively, we identify 1405 novel transcribed regions; 684 of these appear to be new exons of neighboring, often distant, genes. Sixty-four percent of genes are expressed detectably in at least one line, but only 21\% are detected in all lines. Each cell line expresses, on average, 5885 genes, including a common set of 3109. Expression levels vary over several orders of magnitude. Major signaling pathways are well represented: most differentiation pathways are "off" and survival/growth pathways "on." Roughly 50\% of the genes expressed by each line are not part of the common set, and these show considerable individuality. Thirty-one percent are expressed at a higher level in at least one cell line than in any single developmental stage, suggesting that each line is enriched for genes characteristic of small sets of cells. Most remarkable is that imaginal disc-derived lines can generally be assigned, on the basis of expression, to small territories within developing discs. These mappings reveal unexpected stability of even fine-grained spatial determination. No two cell lines show identical transcription factor expression. We conclude that each line has retained features of an individual founder cell superimposed on a common "cell line" gene expression pattern.},
	keywords = {Animals, Cell Line, Cluster Analysis, Drosophila melanogaster, Exons, Female, Gene Expression Profiling, Genetic Variation, Male, Molecular Sequence Data, Signal Transduction, Transcription Factors, Transcription, Genetic},
	issn = {1549-5469},
	doi = {10.1101/gr.112961.110},
	author = {Cherbas, Lucy and Willingham, Aarron and Zhang, Dayu and Yang, Li and Zou, Yi and Eads, Brian D and Carlson, Joseph W and Landolin, Jane M and Kapranov, Philipp and Dumais, Jacqueline and Samsonova, Anastasia and Choi, Jeong-Hyeon and Roberts, Johnny and Davis, Carrie A and Tang, Haixu and van Baren, Marijke J and Ghosh, Srinka and Dobin, Alexander and Bell, Kim and Lin, Wei and Langton, Laura and Duff, Michael O and Tenney, Aaron E and Zaleski, Chris and Brent, Michael R and Hoskins, Roger A and Kaufman, Thomas C and Andrews, Justen and Graveley, Brenton R and Perrimon, Norbert and Celniker, Susan E and Gingeras, Thomas R and Cherbas, Peter}
}
