@article {761796,
	title = {Conserved regulators of nucleolar size revealed by global phenotypic analyses.},
	journal = {Sci Signal},
	volume = {6},
	number = {289},
	year = {2013},
	month = {2013 Aug 20},
	pages = {ra70},
	abstract = {Regulation of cell growth is a fundamental process in development and disease that integrates a vast array of extra- and intracellular information. A central player in this process is RNA polymerase I (Pol I), which transcribes ribosomal RNA (rRNA) genes in the nucleolus. Rapidly growing cancer cells are characterized by increased Pol I-mediated transcription and, consequently, nucleolar hypertrophy. To map the genetic network underlying the regulation of nucleolar size and of Pol I-mediated transcription, we performed comparative, genome-wide loss-of-function analyses of nucleolar size in Saccharomyces cerevisiae and Drosophila melanogaster coupled with mass spectrometry-based analyses of the ribosomal DNA (rDNA) promoter. With this approach, we identified a set of conserved and nonconserved molecular complexes that control nucleolar size. Furthermore, we characterized a direct role of the histone information regulator (HIR) complex in repressing rRNA transcription in yeast. Our study provides a full-genome, cross-species analysis of a nuclear subcompartment and shows that this approach can identify conserved molecular modules.},
	keywords = {Cell Nucleolus, DNA, Fungal, DNA, Ribosomal, Genes, Fungal, Genes, rRNA, Histones, RNA Polymerase I, RNA, Fungal, RNA, Ribosomal, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription, Genetic},
	issn = {1937-9145},
	doi = {10.1126/scisignal.2004145},
	author = {Neum{\"u}ller, Ralph A and Gross, Thomas and Samsonova, Anastasia A and Vinayagam, Arunachalam and Buckner, Michael and Founk, Karen and Hu, Yanhui and Sharifpoor, Sara and Rosebrock, Adam P and Andrews, Brenda and Winston, Fred and Perrimon, Norbert}
}
