@article {761786, title = {Core small nuclear ribonucleoprotein particle splicing factor SmD1 modulates RNA interference in Drosophila.}, journal = {Proc Natl Acad Sci U S A}, volume = {110}, number = {41}, year = {2013}, month = {2013 Oct 8}, pages = {16520-5}, abstract = { RNAi is an evolutionarily conserved gene regulatory process that operates in a wide variety of organisms. During RNAi, long double-stranded RNA precursors are processed by Dicer proteins into \~{}21-nt siRNAs. Subsequently, siRNAs are incorporated into the RNA-induced silencing complexes (RISCs) that contain Argonaute-family proteins and guide RISC to target RNAs via complementary base pairing, leading to posttranscriptional gene silencing. Select pre-mRNA splicing factors have been implicated in RNAi in fission yeast, worms, and flies, but the underlying molecular mechanisms are not well understood. Here, we show that SmD1, a core component of the Drosophila small nuclear ribonucleoprotein particle implicated in splicing, is required for RNAi and antiviral immunity in cultured cells and in vivo. SmD1 interacts with both Dicer-2 and dsRNA precursors and is indispensable for optimal siRNA biogenesis. Depletion of SmD1 impairs the assembly and function of the small interfering RISC without significantly affecting the expression of major canonical siRNA pathway components. Moreover, SmD1 physically and functionally associates with components of the small interfering RISC, including Argonaute 2, both in flies and in humans. Notably, RNAi defects resulting from SmD1 silencing can be uncoupled from defects in pre-mRNA splicing, and the RNAi and splicing machineries are physically and functionally distinct entities. Our results suggest that Drosophila SmD1 plays a direct role in RNAi-mediated gene silencing independently of its pre-mRNA splicing activity and indicate that the dual roles of splicing factors in posttranscriptional gene regulation may be evolutionarily widespread. }, keywords = {Animals, Blotting, Northern, Cell Line, Drosophila, Immunoprecipitation, RNA Interference, RNA Precursors, RNA, Small Interfering, snRNP Core Proteins}, issn = {1091-6490}, doi = {10.1073/pnas.1315803110}, author = {Xiong, Xiao-Peng and Kurthkoti, Krishna and Chang, Kung-Yen and Lichinchi, Gianluigi and De, Nabanita and Schneemann, Anette and MacRae, Ian J and Rana, Tariq M and Perrimon, Norbert and Zhou, Rui} }