@article {761726,
	title = {A rapid genome-wide microRNA screen identifies miR-14 as a modulator of Hedgehog signaling.},
	journal = {Cell Rep},
	volume = {7},
	number = {6},
	year = {2014},
	month = {2014 Jun 26},
	pages = {2066-77},
	abstract = {
	MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression by binding to sequences within the 3{\textquoteright} UTR of mRNAs. Because miRNAs bind to short sequences with partial complementarity, target identification is challenging. To complement the existing target prediction algorithms, we devised a systematic "reverse approach" screening platform that allows the empirical prediction of miRNA-target interactions. Using Drosophila cells, we screened the 3{\textquoteright} untranslated regions (3{\textquoteright} UTRs) of the Hedgehog pathway genes against a genome-wide miRNA library and identified both predicted and many nonpredicted miRNA-target interactions. We demonstrate that miR-14 is essential for maintaining the proper level of Hedgehog signaling activity by regulating its physiological target, hedgehog. Furthermore, elevated levels of miR-14 suppress Hedgehog signaling activity by cotargeting its apparent nonphysiological targets, patched and smoothened. Altogether, our systematic screening platform is a powerful approach to identifying both physiological and apparent nonphysiological targets of miRNAs, which are relevant in both normal and diseased tissues.

},
	keywords = {3{\textquoteright} Untranslated Regions, Animals, Down-Regulation, Drosophila, Genome, Hedgehog Proteins, MicroRNAs, Signal Transduction},
	issn = {2211-1247},
	doi = {10.1016/j.celrep.2014.05.025},
	author = {Kim, Kevin and Vinayagam, Arunachalam and Perrimon, Norbert}
}
