@article {760716,
	title = {Highly efficient Cas9-mediated transcriptional programming.},
	journal = {Nat Methods},
	volume = {12},
	number = {4},
	year = {2015},
	month = {2015 Apr},
	pages = {326-8},
	abstract = {
	The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9. We demonstrate its utility in activating endogenous coding and noncoding genes, targeting several genes simultaneously and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs).

},
	keywords = {Cell Differentiation, Endonucleases, Genetic Techniques, HEK293 Cells, Humans, Induced Pluripotent Stem Cells, Neurons, RNA, Guide, Staphylococcus aureus, Transcriptional Activation},
	issn = {1548-7105},
	doi = {10.1038/nmeth.3312},
	author = {Chavez, Alejandro and Scheiman, Jonathan and Vora, Suhani and Pruitt, Benjamin W and Tuttle, Marcelle and P R Iyer, Eswar and Lin, Shuailiang and Kiani, Samira and Guzman, Christopher D and Wiegand, Daniel J and Ter-Ovanesyan, Dmitry and Braff, Jonathan L and Davidsohn, Noah and Housden, Benjamin E and Perrimon, Norbert and Weiss, Ron and Aach, John and Collins, James J and Church, George M}
}
