@article {760621, title = {Drosophila melanogaster homologs of the raf oncogene.}, journal = {Mol Cell Biol}, volume = {7}, number = {6}, year = {1987}, month = {1987 Jun}, pages = {2134-40}, abstract = { A murine v-raf probe, representing the kinase domain, was used to identify two unique loci in Drosophila melanogaster DNA. The most closely related to v-raf was mapped by in situ hybridization to position 2F5-6 (Draf-1) on the X chromosome, whereas the other raf-related gene (Draf-2) was found at position 43A2-5 on chromosome 2. The nucleotide and amino acid homologies of Draf-1 to the kinase domain of v-raf are 61 and 65\%, respectively. The large amount of a 3.2-kilobase Draf-1 transcript detected in eggs as a maternal message decreases during embryonic development, and significant steady-state levels are observed throughout the remainder of morphogenesis. We speculate that the Draf-1 locus plays an important role in early embryogenesis. }, keywords = {Amino Acid Sequence, Animals, Base Sequence, Chromosome Mapping, Cloning, Molecular, DNA Restriction Enzymes, Drosophila melanogaster, Genes, Mice, Nucleic Acid Hybridization, Oncogenes, Plasmids, Protein Kinases, Sequence Homology, Nucleic Acid}, issn = {0270-7306}, author = {Mark, George E and MacIntyre, Ross J and Digan, Mary E and Ambrosio, Linda and Perrimon, Norbert} }