@article {760061,
	title = {A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.},
	journal = {Genome Biol},
	volume = {8},
	number = {9},
	year = {2007},
	month = {2007},
	pages = {R203},
	abstract = {Off-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based whole-genome RNAi screens for the Wingless and Hedgehog signaling pathways using second generation double-stranded RNA libraries. Furthermore, we investigate other factors that may influence the outcome of such screens, including cell-type specificity, robustness of reporters, and assay normalization, which determine the efficacy of RNAi-knockdown of target genes.},
	keywords = {Animals, Drosophila, Drosophila Proteins, Gene Expression Regulation, Gene Library, Genes, Insect, Genetic Techniques, Genome, Models, Genetic, RNA, RNA Interference, RNA, Double-Stranded, Sensitivity and Specificity, Transcription, Genetic},
	issn = {1474-760X},
	doi = {10.1186/gb-2007-8-9-r203},
	author = {Dasgupta, Ramanuj and Nybakken, Kent and Booker, Matthew and Mathey-Prevot, Bernard and Gonsalves, Foster and Changkakoty, Binita and Perrimon, Norbert}
}
