@article {760026, title = {Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila.}, journal = {Genetics}, volume = {131}, number = {3}, year = {1992}, month = {1992 Jul}, pages = {643-53}, abstract = {We describe an efficient method for generating female germline mosaics by inducing site-specific homologous mitotic recombination with a yeast recombinase (FLP) which is driven by a heat shock promoter. These germline mosaics are produced in flies heterozygous for the agametic, germline-dependent, dominant female sterile (DFS) mutation ovoD1, where only flies possessing germline clones are able to lay eggs. This method, the "FLP-DFS" technique, is very efficient because more than 90\% of females with germline clones can be recovered. We show that this heat-inducible, site-specific mitotic recombination system does not affect viability and that the germline clones recovered are physiologically the same as those created by X-ray induced mitotic recombination. We describe the parameters of FLP-recombinase induced germline mitotic recombination and the use of the "FLP-DFS" technique to analyze the maternal effect of X-linked zygotic lethal mutations.}, keywords = {Animals, Chimera, Chromosomes, Crosses, Genetic, DNA Nucleotidyltransferases, Drosophila, Female, Genes, Lethal, Genetic Linkage, Germ Cells, Hot Temperature, Mitosis, Mutagenesis, Site-Directed, Recombination, Genetic, Saccharomyces cerevisiae, X Chromosome}, issn = {0016-6731}, author = {Chou, T B and Perrimon, N} }