@article {759941,
	title = {I-SceI endonuclease, a new tool for studying DNA double-strand break repair mechanisms in Drosophila.},
	journal = {Genetics},
	volume = {152},
	number = {3},
	year = {1999},
	month = {1999 Jul},
	pages = {1037-44},
	abstract = {As a step toward the development of a homologous recombination system in Drosophila, we have developed a methodology to target double-strand breaks (DSBs) to a specific position in the Drosophila genome. This method uses the mitochondrial endonuclease I-SceI that recognizes and cuts an 18-bp restriction site. We find that \&gt;6\% of the progeny derived from males that carry a marker gene bordered by two I-SceI sites and that express I-SceI in their germ line lose the marker gene. Southern blot analysis and sequencing of the regions surrounding the I-SceI sites revealed that in the majority of the cases, the introduction of DSBs at the I-SceI sites resulted in the complete deletion of the marker gene; the other events were associated with partial deletion of the marker gene. We discuss a number of applications for this novel technique, in particular its use to study DSB repair mechanisms.},
	keywords = {Animals, Crosses, Genetic, Deoxyribonucleases, Type II Site-Specific, DNA, DNA Repair, Drosophila, Female, Genes, Reporter, Male, Models, Genetic, Molecular Biology, Saccharomyces cerevisiae Proteins},
	issn = {0016-6731},
	author = {Bellaiche, Y and Mogila, V and Perrimon, N}
}
