@article {759751,
	title = {Stringent analysis of gene function and protein-protein interactions using fluorescently tagged genes.},
	journal = {Genetics},
	volume = {190},
	number = {3},
	year = {2012},
	month = {2012 Mar},
	pages = {931-40},
	abstract = {In Drosophila collections of green fluorescent protein (GFP) trap lines have been used to probe the endogenous expression patterns of trapped genes or the subcellular localization of their protein products. Here, we describe a method, based on nonoverlapping, highly specific, shRNA transgenes directed against GFP, that extends the utility of these collections to loss-of-function studies. Furthermore, we used a MiMIC transposon to generate GFP traps in Drosophila cell lines with distinct subcellular localization patterns, which will permit high-throughput screens using fluorescently tagged proteins. Finally, we show that fluorescent traps, paired with recombinant nanobodies and mass spectrometry, allow the study of endogenous protein complexes in Drosophila.},
	keywords = {Animals, Cell Line, Cell Survival, Drosophila, Drosophila Proteins, Embryo, Nonmammalian, Female, Fluorescent Dyes, Gene Order, Gene Silencing, Green Fluorescent Proteins, Multiprotein Complexes, Peptide Elongation Factors, Protein Binding, Protein Interaction Mapping, Recombinant Fusion Proteins, RNA, Small Interfering, Stem Cells},
	issn = {1943-2631},
	doi = {10.1534/genetics.111.136465},
	author = {Neum{\"u}ller, Ralph A and Wirtz-Peitz, Frederik and Lee, Stella and Kwon, Young and Buckner, Michael and Hoskins, Roger A and Venken, Koen J T and Bellen, Hugo J and Mohr, Stephanie E and Perrimon, Norbert}
}
