@article {759666, title = {Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster.}, journal = {Nat Methods}, volume = {5}, number = {1}, year = {2008}, month = {2008 Jan}, pages = {49-51}, abstract = {The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.}, keywords = {Animals, Drosophila melanogaster, Drosophila Proteins, Gene Targeting, Genetic Vectors, RNA Interference}, issn = {1548-7105}, doi = {10.1038/nmeth1146}, author = {Ni, Jian-Quan and Markstein, Michele and Binari, Richard and Pfeiffer, Barret and Liu, Lu-Ping and Villalta, Christians and Booker, Matthew and Perkins, Lizabeth and Perrimon, Norbert} }