@inbook {759646,
	title = {Cas9-based genome editing in Drosophila.},
	booktitle = {Methods Enzymol},
	volume = {546},
	year = {2014},
	month = {2014},
	pages = {415-39},
	abstract = {
	Our ability to modify the Drosophila genome has recently been revolutionized by the development of the CRISPR system. The simplicity and high efficiency of this system allows its widespread use for many different applications, greatly increasing the range of genome modification experiments that can be performed. Here, we first discuss some general design principles for genome engineering experiments in Drosophila and then present detailed protocols for the production of CRISPR reagents and screening strategies to detect successful genome modification events in both tissue culture cells and animals.

},
	keywords = {Animals, Cloning, Molecular, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems, Drosophila, Gene Targeting, Genetic Engineering, Genetic Vectors, Genome, Homologous Recombination, Mutagenesis, RNA, Guide},
	issn = {1557-7988},
	doi = {10.1016/B978-0-12-801185-0.00019-2},
	author = {Housden, Benjamin E and Lin, Shuailiang and Perrimon, Norbert}
}
