@article {1645585,
	title = {Transcriptional and functional motifs defining renal function revealed by single-nucleus RNA sequencing},
	journal = {Proc Natl Acad Sci U S A},
	volume = {119},
	number = {25},
	year = {2022},
	month = {2022 Jun 21},
	pages = {e2203179119},
	abstract = {Recent advances in single-cell sequencing provide a unique opportunity to gain novel insights into the diversity, lineage, and functions of cell types constituting a tissue/organ. Here, we performed a single-nucleus study of the adult Drosophila renal system, consisting of Malpighian tubules and nephrocytes, which shares similarities with the mammalian kidney. We identified 11 distinct clusters representing renal stem cells, stellate cells, regionally specific principal cells, garland nephrocyte cells, and pericardial nephrocytes. Characterization of the transcription factors specific to each cluster identified fruitless (fru) as playing a role in stem cell regeneration and Hepatocyte nuclear factor 4 (Hnf4) in regulating glycogen and triglyceride metabolism. In addition, we identified a number of genes, including Rho guanine nucleotide exchange factor at 64C (RhoGEF64c), Frequenin 2 (Frq2), Prip, and CG1093 that are involved in regulating the unusual star shape of stellate cells. Importantly, the single-nucleus dataset\&nbsp;allows visualization of the expression at the organ level of genes involved in ion transport and junctional permeability, providing a systems-level view of the organization and physiological roles of the tubules. Finally, a cross-species analysis allowed us to match the fly kidney cell types to mouse kidney cell types and planarian protonephridia, knowledge that will help the generation of kidney disease models. Altogether, our study provides a comprehensive resource for studying the fly kidney.},
	keywords = {Animals, Drosophila melanogaster, Drosophila Proteins, Hepatocyte Nuclear Factor 4, Kidney, Malpighian Tubules, Mice, Nerve Tissue Proteins, Regeneration, Sequence Analysis, RNA, Single-Cell Analysis, Stem Cells, Transcription Factors},
	issn = {1091-6490},
	doi = {10.1073/pnas.2203179119},
	url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231607/},
	author = {Xu, Jun and Liu, Yifang and Li, Hongjie and Tarashansky, Alexander J and Kalicki, Colin H and Hung, Ruei-Jiun and Hu, Yanhui and Comjean, Aram and Kolluru, Sai Saroja and Wang, Bo and Quake, Stephen R and Liqun Luo and McMahon, Andrew P and Dow, Julian A T and Perrimon, Norbert}
}
