@article {1625902,
	title = {State-of-the-art CRISPR for in vivo and cell-based studies in Drosophila},
	journal = {Trends Genet},
	year = {2021},
	month = {2021 Dec 18},
	abstract = {For more than 100 years, the fruit fly, Drosophila melanogaster, has served as a powerful model organism for biological and biomedical research due to its many genetic and physiological similarities to humans and the availability of sophisticated technologies used to manipulate its genome and genes. The Drosophila research community quickly adopted CRISPR technologies and, in the 8 years since the first clustered\&nbsp;regularly\&nbsp;interspaced\&nbsp;short\&nbsp;palindromic\&nbsp;repeats (CRISPR) publications in flies, has explored and innovated methods for mutagenesis, precise genome engineering, and beyond. Moreover, the short lifespan and ease of genetics have made Drosophila an ideal testing ground for in vivo applications and refinements of the rapidly evolving set of CRISPR-associated (CRISPR-Cas) tools. Here, we review innovations in delivery of CRISPR reagents, increased efficiency of cutting and homology-directed repair (HDR), and alternatives to standard Cas9-based approaches. While the focus is primarily on in vivo systems, we also describe the role of Drosophila cultured cells as both an indispensable first step in the process of assessing new CRISPR technologies and a platform for genome-wide CRISPR pooled screens.},
	issn = {0168-9525},
	doi = {10.1016/j.tig.2021.11.006},
	author = {Zirin, Jonathan and Bosch, Justin and Viswanatha, Raghuvir and Mohr, Stephanie E and Perrimon, Norbert}
}
