@article {1532178,
	title = {Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila},
	journal = {Nat Commun},
	volume = {11},
	number = {1},
	year = {2020},
	month = {2020 09 08},
	pages = {4483},
	abstract = {The Drosophila lymph gland, the larval hematopoietic organ comprised of prohemocytes and mature hemocytes, has been a valuable model for understanding mechanisms underlying hematopoiesis and immunity. Three types of mature hemocytes have been characterized in the lymph gland: plasmatocytes, lamellocytes, and crystal cells, which are analogous to vertebrate myeloid cells, yet molecular underpinnings of the lymph gland hemocytes have been less investigated. Here, we use single-cell RNA sequencing to comprehensively analyze heterogeneity of developing hemocytes in the lymph gland, and discover previously undescribed hemocyte types including adipohemocytes, stem-like prohemocytes, and intermediate prohemocytes. Additionally, we identify the developmental trajectory of hemocytes during normal development as well as the emergence of the lamellocyte lineage following active cellular immunity caused by wasp infestation. Finally, we establish similarities and differences between embryonically derived- and larval lymph gland hemocytes. Altogether, our study provides detailed insights into the hemocyte development and cellular immune responses at single-cell resolution.},
	keywords = {Animals, Animals, Genetically Modified, Cell Differentiation, Cell Lineage, Drosophila melanogaster, Ectoparasitic Infestations, Gene Expression Profiling, Hematopoiesis, Hemocytes, Host-Parasite Interactions, Lymphoid Tissue, RNA-Seq, Single-Cell Analysis, Transcriptome, Wasps},
	issn = {2041-1723},
	doi = {10.1038/s41467-020-18135-y},
	author = {Cho, Bumsik and Yoon, Sang-Ho and Lee, Daewon and Koranteng, Ferdinand and Tattikota, Sudhir Gopal and Cha, Nuri and Shin, Mingyu and Do, Hobin and Hu, Yanhui and Oh, Sue Young and Lee, Daehan and Vipin Menon, A and Moon, Seok Jun and Perrimon, Norbert and Nam, Jin-Wu and Shim, Jiwon}
}
