@article {1439213,
	title = {In vivo study of gene expression with an enhanced dual-color fluorescent transcriptional timer},
	journal = {Elife},
	volume = {8},
	year = {2019},
	month = {2019 May 29},
	abstract = {Fluorescent transcriptional reporters are widely used as signaling reporters and biomarkers to monitor pathway activities and determine cell type identities. However, a large amount of dynamic information is lost due to the long half-life of the fluorescent proteins. To better detect dynamics, fluorescent transcriptional reporters can be destabilized to shorten their half-lives. However, applications of this approach are limited due to significant reduction of signal intensities. To overcome this limitation, we enhanced translation of a destabilized fluorescent protein and demonstrate the advantages of this approach by characterizing spatio-temporal changes of transcriptional activities in . In addition, by combining a fast-folding destabilized fluorescent protein and a slow-folding long-lived fluorescent protein, we generated a dual-color transcriptional timer that provides spatio-temporal information about signaling pathway activities. Finally, we demonstrate the use of this transcriptional timer to identify new genes with dynamic expression patterns.},
	issn = {2050-084X},
	doi = {10.7554/eLife.46181},
	author = {He, Li and Binari, Richard and Huang, Jiuhong and Falo-Sanjuan, Julia and Perrimon, Norbert}
}
