@article {1328525,
	title = {Efficient proximity labeling in living cells and organisms with TurboID},
	journal = {Nat Biotechnol},
	year = {2018},
	month = {2018 Aug 20},
	abstract = {Protein interaction networks and protein compartmentalization underlie all signaling and regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a new approach to study the spatial and interaction characteristics of proteins in living cells. However, current PL methods require over 18 h of labeling time or utilize chemicals with limited cell permeability or high toxicity. We used yeast display-based directed evolution to engineer two promiscuous mutants of biotin ligase, TurboID and miniTurbo, which catalyze PL with much greater efficiency than BioID or BioID2, and enable 10-min PL in cells with non-toxic and easily deliverable biotin. Furthermore, TurboID extends biotin-based PL to flies and worms.},
	issn = {1546-1696},
	doi = {10.1038/nbt.4201},
	author = {Branon, Tess C and Bosch, Justin A and Sanchez, Ariana D and Udeshi, Namrata D and Svinkina, Tanya and Carr, Steven A and Feldman, Jessica L and Perrimon, Norbert and Ting, Alice Y}
}
