@article {1326394,
	title = {Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in cells},
	journal = {Elife},
	volume = {7},
	year = {2018},
	month = {2018 07 27},
	abstract = {Genome-wide screens in cells have offered numerous insights into gene function, yet a major limitation has been the inability to stably deliver large multiplexed DNA libraries to cultured cells allowing barcoded pooled screens. Here, we developed a site-specific integration strategy for library delivery and performed a genome-wide CRISPR knockout screen in S2R+ cells. Under basal growth conditions, 1235 genes were essential for cell fitness at a false-discovery rate of 5\%, representing the highest-resolution fitness gene set yet assembled for , including 407 genes which likely duplicated along the vertebrate lineage and whose orthologs were underrepresented in human CRISPR screens. We additionally performed context-specific fitness screens for resistance to or synergy with trametinib, a Ras/ERK/ETS inhibitor, or rapamycin, an mTOR inhibitor, and identified key regulators of each pathway. The results present a novel, scalable, and versatile platform for functional genomic screens in invertebrate cells.},
	issn = {2050-084X},
	doi = {10.7554/eLife.36333},
	author = {Viswanatha, Raghuvir and Li, Zhongchi and Hu, Yanhui and Perrimon, Norbert}
}
